Shibo Bai

4 Questions 4 Answers 0 Followers

Questions related from Shibo Bai

What things have a high A280 value except for protein typically?

Recently, I am doing preparative SEC for the purification of a protein. I found that the A280 value usually spikes at a very high value when the elution volume is close to 1 column volume....

10 October 2022 8,651 2 View

A wet agar plate would look like what?

recently I got some problems with my spreading of agar plates. 1. The first plate on 7.8 got nothing in PCR (we did a clone transformation) but I think the major problem is from ligation....

28 July 2022 6,499 0 View

What was worng with my colony PCR?

Guys, I just got a problem with my colony PCR. The DNA template I used is ~1.3kbp and it's cDNA in a plasmid with AmpR as my supervisor said. (There is not enough of this DNA template so I want to...

11 July 2022 5,379 6 View

How do we decide whether full-length (FL) tau or repeated domain (RD) tau to express in cell in experiment?

I am just a beginner in tau protein and when I was reading relative papers, I found some experiments express FL tau but others only express RD tau in cells. I got a bit confused, and wondering...

14 January 2022 2,744 2 View