In order to test the effect of a molecule (A) on metabolic patterns, I analyzed changes of ECAR and OCR in neurons and astrocutes. In neurons, A augmented ECAR but had no effect on OCR. However, in astrocytes, A both increased ECAR and OCR. Can anyone give me some iedas about this results. I am very confused. Thanks in advance.
I’m not a specialist, but a number of differences have been reported in neuronal metabolism, as compared with glia (see e.g. Magistretti PJ, Allaman I. A cellular perspective on brain energy metabolism and functional imaging. Neuron. 86: 883-901, 2015). There is also interaction among glia and neurons as far as metabolism is concerned (e.g. Sonnay S et al. How Energy Metabolism Supports Cerebral Function: Insights from (13)C Magnetic Resonance Studies In vivo. Front Neurosci. 11: 288, 2017). I suggest you will need to dig deeper and, perhaps, examine both purified and mixed cell cultures as well as tissue samples to find out about effects and mechanisms of action of your compound.
Rafael Linden Thanks for your suggestion. I agree with your opinion. The differential responses of neurons and astrocytes to a same stimuli may be due to their own differences in metabolic preferentials. But how this differences cause this phenomenon needs further attention. With the papers you suggested read, I've got some ideas. Maybe sometime I will share the results and we can discuss this later. Thanks again.
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