Hi,

I saw there are huge currents during whole-cell sodium current recording in isolated cardiomyocytes. Please see the attached image.

I used a axon 700b amplifier for whole-cell sodium current recording. The resistance compensation alwasys causes these huge currents. These currents can disappear without resistance compensation, suggesting these huge currents are caused by the resistance compensation. I also found that when the resistance compensation decreases from a high percentage to lower, these huge currents will show up when the holding potential is less negative. Please notes that I don't do capacitance compensation in the recording.

Does anyone have idea about this issue? I'll appreciate your help!