Xiaobo Wu

7 Questions 11 Answers 0 Followers

Questions related from Xiaobo Wu

A patch clamping question: why does the resistance compensation cause a huge current at the beginning?

Hi, I saw there are huge currents during whole-cell sodium current recording in isolated cardiomyocytes. Please see the attached image. I used a axon 700b amplifier for whole-cell sodium current...

20 March 2025 5,094 1 View

Is capacitance compensation necessary for sodium current recording?

I do not use capacitance compensation at all for sodium current recording in isolated cardiomyocytes. Only series resistance compensation is used. Therefore, my recording starts with a large...

25 March 2024 1,235 0 View

Why cannot my sodium current return to the max value after recovery from inactivation protocol?

I conducted the recovery from inactivation protocol to record INa in isolated cardiomyocytes. The very strange thing is that the sodium current couldn't return to the max value (marked 1). The...

15 March 2024 9,605 14 View

How to use custom function for curve fitting in clampfit?

Hi, I need an exponential equation with two time constants to fit a sodium current tracing. You can find two attached images: the equation I need is Two exponentials and the red tracing which is...

25 January 2024 3,711 1 View

Is it good to stop negative pressure to increase sealing resistance during patch clamp?

When I gave the negative pressure to seal the membrane, I found that if I released negative pressure during sealing, the sealing resistance increases quickly to reach above 1 gigaohms. Is that...

11 April 2023 2,843 1 View

Do I have to use adhesive substrate for isolated cardiomyocytes during patch clamping?

It looks like laminin or something else is always used as the adhesive substrate for isolated cardiomyocytes. I am wondering if it is also OK to do patch clamping on cardiomyocytes without...

06 April 2023 8,369 0 View

How long do you spend in the training of isolating cardiomyocytes and operating patch clamp?

I have been training myself in cardiomyocyte isolation and patch clamp operation for almost two months. Our lab doesn't work in electrophysiology, so I learned from different labs. Completing both...

09 March 2023 9,485 1 View