Histones are abundant proteins, so how many (% per total/proportion/number per total/number per cell, etc - however you'd like to express the proportion) of those have trimethylation of histone H3 lysine 4? Thanks
In terms of genome coverage (which is an important factor in regards to your question), around 1-3% of the genome (maximum) is covered with H3K4me3 according to ChIP-seq. Usually with ChIP-qPCR, of the H3K4me3 positive regions, DNA recovery is usually 5-50% of input. So I would say only a small proportion of H3 in chromatin has H3K4me3. When we do ChIP- we usually recover quite little DNA for this Hmod even though the antibodies and ChIP-seq works best for this Hmod (hence it is commonly used as a positive control for histone ChIPs).
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