Some Oxford Nanopore kits have Primers with 5 ' modification that provide ligation to adapters without the need for ligases (ligase-free method), as in 16S barcoding kits. Does anyone know what modification this is or the chemistry involved in the process? I'm very grateful if anyone can help me with this information. Thanks.
Xuhua Wang
Company's top secret! You may be bound to the licence when you bought their product.
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Callum Parr
I am guessing some kind of click chemistry so some kind of amide or alkyl group that would then be complementary to group on what you attach to.
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