A good reference for polyphasic identification of Acremonium-like species?

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How long to treat male mouse to affect the offspring ? When is the paternal programmation efficient ?

My question is mostly about how many spermatogenesis cycle does it take so all the spermatozoa are effected by a deficiency / treatment, and so the offspring is completely affected ? No matter if...

10 January 2024 7,099 0 View

What is the 70kDa band in Western blot of full mouse heart?

Dear I performed Western blot on full mouse heart lysates of MHC-cre mice. I tested 2 different genes, and in both Western blots I had an unexpected band at 70kDa. Does someone has an explanation...

16 November 2023 7,864 0 View

How long does it takes to digest ~20mg of tissue with Proteinase K at 56°c ?

I'm preparing DNA extractions after digesting my 345 samples with PK, but I'm wondering how long i'll take to digest each tissue If anyone has a precise idea about how long will it takes, within a...

02 August 2023 6,565 1 View

Is it possible to display, percentage of limitation speed reached on PVT Vissim ?

Usually when a speed map is displayed on PVT VISSIM 21, a color is associated to each link depending on the average/maximum/minimum speed on this link. Is there a way to display a color depending...

03 April 2023 8,921 0 View

Is it possible to stain cells after flowcytometry?

Hi all! I'm purifying endothelial cells with FACS, but to confirm the cell population after FACS, I want to stain the sorted cells to visualize them under the microscope. So far, no succes. Does...

08 February 2023 7,389 2 View

Strange immunostaining of P-gp on Caco-2/TC7 cells ?

Hello Scientific Community, I need your help, I did an immunostaining of P-gp on Caco-2/TC7 cell line. I used a classic protocol with 4% PFA fixation and without permeabilization. You can...

01 February 2023 4,000 3 View

What are the best standardized tests to establish cognitive abilities/profile of participants with complete blindness or severe visual impairment?

I work with people with complete blindness, and tasks are cognitively demanding. While I made sure our participants did not present comorbidities and cognitive impairments, I am looking for...

23 January 2023 6,396 4 View

How to perform Western blot on low cell count?

Hello I am working on a protocol to perform Western Blot on sorted endothelial cells from the liver. So far, I tried to do protein isolation in NP-40 buffer with 120.000 cells but I do not get...

07 November 2022 8,543 4 View

Evan's blue dye is a relevant technic to verify the blood brain barrier integrity after viral infection in small rodents?

I would like to examine the neuroinvasive properties of tick-borne encephalitis virus in mice and I do not know if macroscopic observation of the brain using Evan's blue is relevant enough to...

09 June 2022 2,430 0 View

Can the electron mobility be estimated with tunneling spectroscopy?

I'm trying to wrap my head around the fact that graphene has a huge electron mobility and a zero density of states (DOS) at the Fermi energy (EF) – yet some other materials can be known as poor...

15 March 2022 697 0 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Do you know any references for analyzing stochastic fiber orientaion composites ?

Hello everyone I am looking for one or some books for propertes and behaviors of stochastic fibre orientation composites. unfotunately I could not find any suitable reference for thias by...

04 August 2024 3,461 3 View

What is fitofthora instance of potatoes?

Now research fellow used it to prevent fungus in potato,may be potatoes gene effect.

02 August 2024 180 3 View

What is gangrine? Is there gangrine happened indie potato?

Please discuss someone told about fungus and allied others.

31 July 2024 3,372 3 View

Are you interested in contributing a book chapter on biomaterials in Orthopaedics?

I am editing a book for Springer Nature on recent advancements and future trends in biomaterials for orthopedic and trauma applications, with a publication date slated for early next year. If you...

29 July 2024 5,015 9 View

"Idrisi Selva" tutorial Books for pridiction with driving factors in modeling?

Some educational books with practical exercises of the "Idrisi Selva" software, which have step-by-step visual training for predicting the change of land use/land cover and including the use of...

28 July 2024 4,168 0 View

Question about Single Strain Arbuscular Mycorrhiza Fungi??

Now i am working on AM fungi single strain. What are the most effective isolation techniques for obtaining pure cultures of arbuscular mycorrhizal (AM) fungi?? Is there are best approach...

26 July 2024 6,520 1 View

Dear researchers, how can we download this book? Please help?

Several Complex Variables and Integral Formulas Dear researchers, how can we download this book? Please help?

22 July 2024 4,120 4 View

Do anyone knows any book dealing with fish growth/zootechnical performances and somatic indexes in aquaculture?

In the aquaculture and fish nutrition research field, a number of growth and somatic indexes are often used, including specific growth rate (SGR), feed conversion rate (FCR), protein efficiency...

22 July 2024 2,480 4 View

Any recommendations about affordable publishing for a book ?

Asking for recommendations about affordable publishing

17 July 2024 1,555 1 View