Hello everyone. I'll be doing a PCR products clean up and I received the suggestion of using AMPure XP since it's easy to distribute among the wells. I'd like to know if someone knows about a product that can be efficiently used as this one. Thank you in advanced.
It depends on what you are doing next but if it is for sequencing I always liked the exo-sap method ( home bought reagents not kit form is much cheaper) which is very quick (15-20mins) but many of my colleagues found the microclean kit made by Microzone to be quick ( 20 minutes) to be cheaper and very efficient . Both of these are liquid based so pipetting is easy. AMPure is probably slightly quicker but keeping it in suspension may be slightly more difficult and the other methods may be cheaper
Dear Diego
i used several time kit based on 96 well plates using vacum. as_
1) promega wizard sv96
https://ita.promega.com/products/dna-purification-quantitation/high-throughput-nucleic-acid-purification/wizard-sv-96-pcr-clean-up-system/
2) omega biotek EZ-96 cicle pure
http://omegabiotek.com/store/product/e-z-96-cycle-pure-kit/
to purify small pcr fragments to be sent to sequenticng for MLST analisis and generally i obtained good results with both kits.
good luck
Manuele
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