Diego Guerrero

2 Questions 3 Answers 0 Followers

Questions related from Diego Guerrero

How can you calculate how many samples can you get into a single cartridge for Miseq V3 sequencing? (600 cycles)

I'm trying to sequence amplicons of the 16S V3-V4 region (aprox. 540 bp). I've read that at least you need 10,000 reads per sample to characterize a microbial community. If I'm expecting at least...

14 June 2017 587 2 View

96-well plate PCR products clean up?

Hello everyone. I'll be doing a PCR products clean up and I received the suggestion of using AMPure XP since it's easy to distribute among the wells. I'd like to know if someone knows about a...

28 April 2017 976 3 View