Good day! Please tell me the resources with which you can build 3D models of the protein structure, knowing the amino acid sequence?
Thanks!
Hi Mr. Voroshilin,
I recommend I-TASSER and Alphafold for structure modelling and prediction.
SWISS-MODEL (expasy.org), this is an online modeling program.
Dear Roman Voroshilin
it depends from the properties of your protein sequence.
IF you find in the PDB (performing a BLAST in PDB a protein with structure solved with high homology (>30-40%)
you can find an example of BLAST on PDB at the 7' 18'' on the following link:
https://www.blogger.com/video.g?token=AD6v5dyPGWsbPCAGPM4bhC7qrFmUL9TIfeArxzSqZ-ao_K6iC3z597YtqkSB2QIvBIxy9TVU52O9By7lhSuO-TFmnmd1-xRgRxnhW6uEon8lV-Lk-zMV2mZq1MT0o4gwaT38-wIRIIY
MODELLER or Swiss-model are the best approach.
If you do not have any good template, Rosetta or I-tasser (which do not need a full templates) are alternative
At the 11' of the previous link, you can found also information about it.
good luck
Manuele
DEAR Roman Voroshilin
Please consider these references such as:
1-Protein structures for all, DOI:10.1126/science.acz9822
2-Protein structure prediction,DOI:10.1016/B978-0-323-89775-4.00023-7
3-Protein structure prediction
Dear Roman Voroshilin,
Knowing the amino acid sequence and after downloading a software for the visualization of the structure, like Pymol, you can easily get structural predictions through these free servers:
- Robetta (http://old.robetta.org/fragmentsubmit.jsp)
- trRosetta (https://yanglab.nankai.edu.cn/trRosetta/)
- RaptorX (http://raptorx.uchicago.edu/)
Good luck!
Thank you all for your valuable advice!
try AlphaFold
https://alphafold.ebi.ac.uk/
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