Hi Everyone

I am Graduate student who isnt the greatest at statistics and I have a question regarding a 3 way ANOVA. I am trying to run a 3-way ANOVA for a data set in graphpad that is the following:

-3 diets (X,Y,Z)

-3 Genotypes (W, H, K)

-high fat vs low fat

In total, I have 9 groups (3x3 diet/gene interactions), between both high and low fat fed animals, and I set up the graphpad sheet exactly how it displays on the website, however, I get an error saying that the data is not correct to run a 3-way. Really my questions is that is this a limitation in graphpad, or can I only run a 3-way ANOVA with 4 groups? (PS if i trim my groups to 4 in graphpad I am able to run a 3-way.

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