I'm struggling to understand the question. However, if you mean that you have identified the correct toxin from the bacteria(I assume from another means) then can you confirm the taxonomic classification of the bacteria based on the amplification of a univesal primer?

You could not be 100% sure. As its a universal primer it is created to amplify the 16s in upper echelons of taxnomic classification. If the universal primer is

that broad then potentially the expression of that toxin could also be widley expressed.

Unless you know that only this bacteria produces this toxin and no other closely related species do, then you have some degree of assurance.

Probably still best to get is sequenced.

Thanks alot for yor prompt reply. let me explain i already sequenced data and genus level is same but variation of spp level.

my bacteria is clostridium perfringens and its confirmed By toxin and 16s primer confirm genus level .

I would recommend to use other markers besides 16S, that can increase the certainty on the microorganism identification. Some of the markers used for that are rpoB, cpn60, tuf, rp operon. In International Journal of Systematic and Evolutionary Microbiology there are many example of studies making a good identification of bacteria.

thanks for reply can you plz share,any article regarding biomarkers regarding clostridium perfringens . will be grateful to you for this act of kindness.

thanks for reply can you plz share,any article regarding biomarkers regarding clostridium perfringens . will be grateful to you for this act of kindness.