During C –TAB phenol chloroform based jellyfish DNA isolation procedure I have confused with a step for buffer preparation. Any one helps me to clarify the required amount of NaOH for 0.02M EDTA (pH 8). In addition to this, please help with details about the C-TAB Phenol-Chloroform chemical preparation for jellyfish .Now I am following modified CTAB Phenol-Chloroform method (Dawson et al. 1998).
Dear Riyas Abdul,
Weigh 3.723G of EDTA sodium salt and transfer it 1000ml standard flask and then fill it up to 1000ml mark and mix it thoroughly
I have also attached the link to the file
Hope this will solve your problem
http://nitttrc.ac.in/Four%20quadrant/eel/Quadrant%20-%201/exp5_pdf.pdf
Well, CTAB buffer will require very small amounts of 0,02M EDTA in final solution so preparing 1 litre will be so high, it will also have a short expire date after preparation.
I suggest you to prepare 54 ml with 0,2 mg EDTA (but you should check your owns moelcular weight, I used 292g/mol) than add half of a NaOH tablet or KOH.
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