Im trying to germinate Nitrate Reductase Arabidopsis mutants (nia1nia2) in 0.5 MS media (in plate with agar 1%). I didn't find a specific protocol to germinate these plants, but I tried to vernalizate them with GAs 1mM (for 24h and for 5 days) and nothing happened.
Am I missing something? (MS has nitrate, but also ammonium so nitrogen source shouldn't be a problem). Should I consider the problem is with the seeds?
Andrew Paul McKenzie Pegman
Test for seed viability first either with tetrazolium or flotation in water (viable seeds sink). Then just use viable seeds :)
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Andrew Paul McKenzie Pegman
It's a palm tree, which are not cycads, but angiosperms. Cycads are gymnosperms :)
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