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Hi all, I'm using the plasmid pPlatTET-gRNA2 (addgene, #82559) to try and hypermethylate some target regions. I want to target four different regions, and hence had four different gNRAs. To...
17 July 2025 3,432 6 View
Relationship between distributed leadership and school improvement outcome.
17 July 2025 6,687 6 View
Moreover, this question seeks to challenge and, therefore, advances PhD candidates to reflect on the meaning and consequences of their research beyond the realm of mathematics. It asks how that...
10 July 2025 9,614 2 View
We used CRISPR-Cas9 to create mutant zebrafish by injecting it into the eggs of wild-type (WT) fish at the one-cell stage. After letting them grow for three months, we cut their fins, extracted...
10 July 2025 4,006 3 View
I carried out a DNA extraction with purification using magnetic beads. The eluate had a lot of bead residue. We removed the beads, placed the samples on an agarose gel and observed genomic DNA...
09 July 2025 2,652 4 View
Hello, we do isolation DNA from horse hair (20-30 hair), however our concentration/ yield is still low is low even when we using DTT solution. When we measure the extracted DNA on Qubit, the...
09 July 2025 2,248 5 View
We have identified a few G-quadruplex DNA aptamers in our lab against a specific target. We would like to build models of the identified aptamers using some software. We modeled them using...
09 July 2025 7,704 2 View
Estoy trabajando en un estudio sobre exclusión social en barrios vulnerables y me interesa conocer experiencias o enfoques que hayan resultado útiles en investigaciones anteriores. ¿Qué técnicas...
09 July 2025 3,819 0 View
A hot discussion that requires your valuable insights.
09 July 2025 4,515 5 View
Hi, We recently purchased a Fluostar Omega plate reader and were taken through the installation and basic setup including the installed optics. I'm now trying to write an SOP with pictures of the...
09 July 2025 8,284 0 View
The impact of educational policy from state to states.
08 July 2025 7,920 3 View
Hello, My question is related to use and processing of multiple MODIS products such as MOD17A2HG (GPP and PSN), MOD16A2HG (ET and PET) and MOD11A2 (LST). Each MODIS product has its own QC...
08 July 2025 5,529 0 View
I have a panel data of N=23 and T=30 I’m a bit confused about the Pesaran CD test. Could someone please clarify whether the "Pesaran CD test" and the "Pesaran scaled LM test" are exactly the same,...
07 July 2025 2,574 4 View
We prepared a Cu-based photocathode for PEC water splitting. We measure a photocurrent density as well as O₂ gas evolution. However, we couldn't obtain H₂ gas evolution. But, reference papers...
07 July 2025 4,548 1 View
I tested the EIS of layered oxide materials for sodium batteries (coin cell). EIS data shows that the ohmic impedance and charge transfer impedance decrease after 200 cycles, compared with the EIS...
06 July 2025 7,150 2 View
I am extracting an analyte that perciptated to form light weight crystal like particles. Almost looks like fine table salt (or white creatine crystals). If the analyte is low in concentration,...
06 July 2025 8,693 4 View
Molecular docking technique is used to assess effect of 1 drug molecule i.e. ligand on protein active site identified as causal factor behinds a particular disease. Synergistic effect in...
03 July 2025 4,269 3 View
Information on solar irradiance, Energy sustainability for a 3 room apartment running in an off grid state with battery energy storage, weather conditions
03 July 2025 4,306 3 View
However we feel about fantasies, it is clear that sex is not, for most of us, just a rubbing together of bodies, but involves our minds … We take our minds with us into each sexual encounter....
01 July 2025 5,497 0 View
So I'm working on a project in that I require a compact load cell which is small, has load bearing capacity upto 200kgs and gives accurate results. Please provide the procedure step by step and...
01 July 2025 1,500 2 View
I am a 76-year-old retiree trying to use ANSYS 2025R1. I wish to find the nearest node to some specific (X,Y,Z) locations. All I read on the Internet is to use the "nearest_node=Node(X,Y,Z)"...
29 June 2025 3,625 4 View
Should teachers give harsh feedback to prepare students for the real world or soften it to encourage them?
29 June 2025 7,939 11 View
Does anyone have updated data on the probability of upcoming volcanic eruptions (excluding supervolcanoes)? I am currently observing increased volcanic activity indicators, similar to the...
27 June 2025 3,425 1 View
Why would oppressive regimes prioritize weapons instead of the people's welfare?
24 June 2025 5,207 2 View