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Cloning EGFP to pet28a - Need Help Troubleshooting - where is the error?

Hello everyone, I recently cloned EGFP into pET-28a(+) and transformed it into BL21(DE3) cells. However, despite trying multiple conditions, I am not observing any yellow-green color or...

10 February 2025 8,587 8 View

What are the comparative advantages and limitations of emerging optimization techniques in the context of renewable power integration?

The increasing integration of renewable energy sources (RES) into power grids presents significant challenges due to their inherent variability and unpredictability. Traditional optimization...

09 February 2025 7,571 2 View

How to calculate cells.g−1?

I have 20 days and 9 hours left and I need to know. How to calculate cells.g−1? 200mg of dry sediment was suspended in 9.8 ml of MGW and 100ul was plated. How to calculate CFU/ml (no dilution)...

09 February 2025 2,447 1 View

Changing properties (density and dynamic viscosity) of molten salt mixture in Aspen plus?

I am working with a molten salt mixture that is solid at room temperatures but in the system operates exclusively in the liquid state. In Aspen Plus, I use the databank to define the components,...

05 February 2025 7,421 2 View

What is the role of thin sections in studying mineral recrystallization caused by heat and pressure generated during earthquakes?

I think with some paper discuss about thin sections are an essential tool for studying mineral recrystallization caused by earthquakes. They provide detailed insights into the microstructural and...

02 February 2025 7,387 2 View

How can i write research paper on mechanical drawing topic?

tell me how do i write research paper on mechanical drawing topic

02 February 2025 7,906 1 View

How to register new institution or foundation beside campus?

I have a foundation that it is work on research and development. some of junior researcher that did not work on formal institution is working at my research foundation. they want to register their...

01 February 2025 5,989 0 View

How to Properly Implement Viscosity in SPH Using ABAQUS Explicit Analysis?

Hello everyone, I have a question regarding the use of viscosity and artificial viscosity in Smoothed Particle Hydrodynamics (SPH) for ABAQUS Explicit dynamic analysis. Here's the setup I'm...

28 January 2025 1,313 0 View

What recent methodologies do you recommend for analyzing manipulation and identity construction in political discourse?

What recent methodologies do you recommend for analyzing manipulation and identity construction in political discourse?

27 January 2025 5,129 2 View

Do you have the archives of M 7.7 Earthquake and Devastated Tsunami in Molucca (Maluku) Indonesia?

Hi All Greetings from Jakarta, Indonesia. I hope you are doing well. Allow me to share with you all that I am currently seeking the documents or archives related to the Indonesia Molucca tsunami...

27 January 2025 5,501 0 View

What is the role of insect biodiversity in maintaining crop health and productivity?

how beneficial insect diversity contributes to pest regulation and pollination services.

26 January 2025 5,294 2 View

Can slope replace topography in calculating (DRASTIC model) for groundwater contamination?

Why is slope sometimes calculated as a percentage instead of degrees and what does a slope number represent as a percentage, for example (2%)? Please provide me with a specific answer and...

24 January 2025 7,298 3 View

Info on Bird tags suppliers in Saudi Arabia??

HI Folks, Can anybody guide me about potential suppliers of the below mentioned bird tags? It does not have to be that brand, the important things are the specs. Nanofix® GEO +RF MINI for...

22 January 2025 3,317 0 View

¿Cuáles son las proyecciones para el comunicador organizacional?

Necesito conocer ¿Cuáles son las proyecciones para el comunicador organizacional? ¿Cuál es el futuro de su perfil? ¿La tecnología influye o influirá en su perfil?

22 January 2025 9,942 0 View

How can a researcher contextualize an instrument for his/her study?

I used 21st century skills scale developed by Ravitz in my study. One of the comments I received from one of the professors was that "you cannot simply use an instrument developed for another...

20 January 2025 721 6 View

How can you maintain your mental, emotional, and physical health when you don't have a job to go to every day?

Taking care of mental, emotional, and physical health is crucial, especially when we don't go to work every day. Whether due to unemployment, retirement, or working from home, focusing on health...

19 January 2025 8,212 0 View

If the mean, median and mode are equal, then the distribution is ?

Short answer

17 January 2025 2,292 0 View

R squared or Chi squared?

So I'm doing a sorption experiment, and I've come to the point where I'm evaluating which of the kinetic models best suits my adsorption process. Since I've used the nonlinear models, should I...

15 January 2025 9,623 3 View

Can anyone review my research proposal on abiotic stress mitigation in plants using nanomaterials?

Hi, I’m Muhammad Mukheed, and I’m working on a research proposal on abiotic stress mitigation in plants using nanomaterials. This builds on my Master's research, where I examined morphological,...

15 January 2025 7,147 0 View

Dipole correction in CASTEP is turned off when performing optimization. How to apply correctly?

I am trying to run a simple CASTEP optimization of a slab of a metallic material with dipole correction in order to get the workfunction value. To do so, i added to the .param file the following...

15 January 2025 5,659 0 View

How does hardware affect model performance?

Hello everyone, I have observed significant variations in model performance when training on different GPUs. For instance, when training on an RTX 3060 compared to an RTX 4090, the performance...

12 January 2025 5,908 4 View

Hi everyone. Can you guide me how to use tonyplot function in silvaco to simulate gate charging in mixedmode?

hi everyone. Can you guide me how to use tonyplot function in silvaco to simulate gate charging in mixedmode?

09 January 2025 5,553 0 View

Super-infection in Operated cases of Hydatid Cyst?

🔵 Topic: Super-infection in Operated cases of Hydatid Cyst Subject: Infectious; Surgery; Type: Original Article Needed: 1 Job: Introduction and Discussion Position: 3rd Deadline: 1 month

09 January 2025 4,813 0 View

How can i create a spiral shape using wier by considering it as beam or truss element?

Give some Reference Paper if possible

09 January 2025 9,781 0 View

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