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Hello, I performed a western blot which I have previously done with the exact same antibodies. However, this time after probing for B-actin control (anti-mouse 2nd) and subsequent washing and...
26 May 2025 9,312 4 View
This study explores whether the combination of mild mitral valve prolapse and orthostatic hypotension is associated with cognitive symptoms such as brain fog and reduced attention. Rather than...
26 May 2025 2,126 0 View
La cultura organizacional tiene un impacto profundo y bidireccional en el diseño y la efectividad de la estructura organizacional. Actúa como un molde invisible que da forma a como se concibe y...
23 May 2025 8,244 3 View
I did a western blot today with a precast gel at 200v for 40 min. Everything was the same to what I have done before but the bands look skewed.
22 May 2025 6,882 0 View
Hi everyone, I am currently facing some problem to model internal pressure inside a pipeline in Flac 3D. Is there any way to doing it? Any help is very much appreciated. Thank you in advance.
22 May 2025 8,415 1 View
I would like to ask for insights regarding the image I uploaded. The background is a scanned cross-polarized light (XPL) photomosaic of a thin section, with cathodoluminescence (CL) images of...
21 May 2025 3,086 2 View
I performed an MTT assay to determine the viability and toxicity values of cells. For analysis of MTT assay results, readings were performed using an ELISA reader. How do I determine the LC50...
21 May 2025 5,722 2 View
I am using the anthrone assay in samples with ethanol to determine total carbohydrates. At the same time, I'm using reduced sugars by the DNS assay. However, when I compared the results, I saw...
21 May 2025 2,444 0 View
I will answer this question in the context of Nigeria. I have provided some discussions below on how to sustain private investment and boost economic growth in Nigeria. The following strategies...
20 May 2025 1,924 5 View
Hi, I am determining pronase activity using a continuous fluorescence-based micro assay with FITC-casein as substrate (25 μg/ml final concentration in the assay). Excitation and emission...
16 May 2025 8,022 5 View
Hello, Assuming a scenario such as: if one collected water samples from 9 different sites in a time series of four months (within two different seasons were each season had two months as a...
13 May 2025 6,472 9 View
There are four samples with a composition of 50% Sn-50% Zn. Different casting methods were applied to all of these samples and as a result, different microstructures emerged. In this case, it is...
13 May 2025 3,120 0 View
Dear fellow Research Gaters, For my qualitative thesis on single-person households, I am looking for articles (demographic; sociological) that include important basic statistical data and...
13 May 2025 1,557 2 View
Dear fellow researchers, As part of my PhD project in Educational Psychology, I am studying the impact of teachers’ psychological health and school organizational health on student academic...
12 May 2025 2,404 1 View
12 May 2025 3,992 2 View
Why vote buying?
09 May 2025 6,978 2 View
I am currently undertaking a quantitative research project that requires the use of SPSS. Usually i do not struggle with this software however this time i am. I am trying to figure out what...
08 May 2025 3,664 4 View
I recently performed a hydrodistillation of plant material in the lab to extract essential oil. However, after the distillation, the oil and water did not form distinct layers. Instead, I observed...
04 May 2025 1,035 1 View
How to detect (wet gravimeteric analysis) the interference of nitrogen-containing compounds while determining the nitrates in hard and drinking waters and how to elimiate such interferences?
02 May 2025 8,238 6 View
If after activation jurkat cells lose viability then how can flow cytometry based assays or cell proliferation assays will be performed ?
30 April 2025 328 1 View
I am currently researching the relationship between cosmopolitanism and urban competitiveness in emerging cities.. Could anyone with expertise in urban studies, economics, or cosmopolitan theory...
30 April 2025 177 3 View
Integrating high interest with an individual's curriculum in a way that student will want to participate in curriculum centered activities.
29 April 2025 3,671 5 View
12 years ago I asked How can we achieve appropriate correlation between functional strategies? And now After 12 years I'm going to ask how can we leverage data analysis and business analysis for...
28 April 2025 4,439 5 View
In my DFT calculations on nanoflakes of 2D quantum dots, I observe a few small negative frequencies in the range of 10–30 cm⁻¹, even after full convergence. I have used both effective core...
26 April 2025 1,135 5 View