I have performed several enzymatic: lipolytic, amylolytic, and proteolytic activity assay.
After looking into it, it turns out there are three formulas for calculating the hydrolytic index.
For example:
D: diameter of hydrolysis zone
d: diameter of bacterial colony
First formula: (D-d/)d
Second formula: D/d
Third formula: (D+d)/d
What is the meaning of these three formulas?
Thanks in advance.