Hi have you tried picking the colony or observe the size of the colonies each day to see whether it’s growing or not? Cuz the first trial looks fine to me. Of course the colony won’t be as putative as the stable iPSC lines, which is why we need to pick and expand it. I think what you did the first time works, maybe just need to let the cells grow a bit. If it can adhere then it’s already a major win.

Ahmad Khairul Aizad Ahmad Khairi Thank you so much for the response