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I am working on antioxidant profiling of medicinal plant extracts, and I aim to assess lipid peroxidation levels induced by oxidative stress in biological systems. Although the TBARS assay (based...
06 August 2025 3,678 6 View
If I want to construct a plasmid containing only the E. coli chromosomal origin of replication fragment and the pUC19 backbone with its antibiotic resistance, how should I proceed? I could first...
05 August 2025 1,604 2 View
Hi! I lost access to my Research Gate account and I've created a new one, but the previous one is still on (with all my contacts and publications...). How should I proceed?
05 August 2025 3,002 1 View
Hello, I saw these clumps of red clumps in my cell lines. I wonder what this is. I am currently culturing lymphoblastoid cell lines with 15 % FBS and 1% Pen Strep.
05 August 2025 6,168 1 View
Topology: What new insights can persistent homology provide in the analysis of high-dimensional data sets?
04 August 2025 8,890 2 View
Hello all, I'm trying to do minigene assays for multiple genes. I got 5 genes to work for the wildtype using pET01 exon trap vector, and the splicing assay worked fine. Now I'm trying to...
04 August 2025 6,525 2 View
Hi I want to electrically stimulate the hippocampus neurons (CA1) and record the field potential in ACC in acute slice. I did not find any paper describing the method of this type of recording....
04 August 2025 2,843 5 View
The world is undergoing rapid changes, accompanied by tremendous technological and information development. With the use of artificial intelligence in agriculture, what does the future hold for us?
04 August 2025 9,845 3 View
The form is addressed to professionals with expertise in branding, marketing, or brand management. It will take less than 10 minutes to complete, and your responses will be treated with strict...
03 August 2025 9,925 3 View
Greetings, Is it possible and scientifically sound to develop and generate hypotheses based on focus group data instead of evidence from the literature? If yes, I would appreciate it if you could...
02 August 2025 3,840 2 View
After WES analysis, I identified genes with SNPs existing in WT (wild type), MT (mutant), and heterozygous forms. I want to clone these variants and express them in cells. 1. How can I clone the...
31 July 2025 9,916 2 View
The markers on the far left are 180 kDa, 130 kDa, 100 kDa, and 75 kDa. The three lanes are control group 1 and treatment group 2, respectively. Each group is separated by a marker. The primary...
31 July 2025 8,648 1 View
How does parental involvement influence the educational progress of children receiving special education services?
31 July 2025 8,884 2 View
I’m doing pei max transfection in platinum E cell lines to collect retrovirus supernatant but my transduction is very low. if I add sodium butyrate as booster for enhancing transfection and...
30 July 2025 4,941 2 View
I performed co-immunoprecipitation (co-IP) with mouse anti-GFP antibody and mouse anti-myc antibody. Non-specific bands appeared in all samples in the co-IP experiment. Non-specific bands were...
28 July 2025 5,532 1 View
I prepared a 1 mM stock solution of Decitabine in DMSO and added 1.32 μL to 4 mL of culture medium (final concentration: 330 nM) to treat Burkitt’s lymphoma cell lines. The cells were cultured...
28 July 2025 981 3 View
How does public discourse against DEI shape the curriculum and teaching materials used in schools?
28 July 2025 7,387 2 View
How to get citation for research papers written in local languages?
26 July 2025 8,664 0 View
I'm performing site-directed mutagenesis using Agilent’s QuickChange kit. My goal is to introduce a single nucleotide substitution to change one amino acid. The primers were designed using...
24 July 2025 665 0 View
I am researching on the various implications that AI led processing will have on the Business Process Outsourcing industry (BPO). 1. What kind of work will be eliminated? 2. What kind of work...
24 July 2025 6,386 5 View
I am currently working on an electrophysiological study of long-term potentiation (LTP) and need to generate a graph showing the normalized slope percentage over time. It is essential that the...
23 July 2025 6,315 5 View
Hi I'm now working on primary mouse neuron and astrocyte co-culture. My problem is that my pre-cultured neurons detach after the astrocyte co-culture.. I used PDL coated glass coverslips. DIV7...
23 July 2025 4,886 4 View
Thought research was a platform to help researchers in need 🤔 I have written to at least 3 researchers pleading for R codes but none gave out.
22 July 2025 8,680 1 View
I'm curious about the current frontier of AI neuroscience. While we've seen remarkable progress in specific tasks, applying AI to truly decipher the brain's intricate mechanisms, especially across...
21 July 2025 6,525 3 View