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Could you please guide me about the protocol for conducting electrophoresis at very low concentrations of DNA (below 1 ng/ul)? I did electrophoresis with two percent agarose gel (+1ul safe stain)...
02 November 2024 2,577 3 View
I have tried using 12% resolving gel as well as 10% resolving gel and 5% stacking gel combination. I have also run the marker which separated fine. But why can I not see bands for my sample? i...
01 November 2024 2,904 4 View
Dear ResearchGate Team, I recently received access to research, but unlike previous times, I couldn’t reach out to the author to thank them (see attached screenshot). I understand from the error...
01 November 2024 3,000 0 View
The National Eligibility cum Entrance Test (NEET) is a national level medical entrance examination in India. It was introduced in 2013 by CBSE as an attempt to unify all the medical entrance...
21 October 2024 8,605 0 View
Hello everyone, I’m currently running a Comet Assay and have encountered an issue where the voltage during electrophoresis can’t go above 10V, even though I’ve set the current to 500 mA and power...
08 October 2024 8,611 0 View
Reducing food waste is a global priority for environmental protection and social well-being. The scientific study 'Food waste: A systemic approach and structural prevention' – published on...
06 October 2024 8,894 2 View
IS THERE ANY ADDED ADVANTAGE OF COMBINING TWO ORGANIC INHIBITORS WITH ALREADY HIGH INHIBITION EFFICIENCIES TO COMBAT A VERY CORROSIVE ENVIRONMENT TO A CERTAIN MATERIAL
01 October 2024 7,943 1 View
Hello everyone, I’m currently working on a Data Envelopment Analysis (DEA) project and I’m unsure whether to use original values or natural logarithms value for my input and output variables. I...
26 September 2024 1,552 2 View
These are a few things that come to my mind. Approach 1 - Highly Cited Papers Read only important research papers (say only highly cited) from the point of doing Mind Mapping to find out narrow...
25 September 2024 515 1 View
How do/what tools researchers used to paraphrase their literature to decrease the similarity scores, after of course mentioning the citations?
21 September 2024 1,400 1 View
I am looking for a database on the volume of US mine tailings and the annual production rate of metallic mines. A paper, report, or even a website, including a graph, works for me. The most...
19 September 2024 6,534 0 View
The name assembly _none has been used on a previous orientation definition. Orientation name must be unique
18 September 2024 6,774 2 View
It seems that it is difficult to find and download some measurment tools such as questionaires.
16 September 2024 3,751 1 View
I have around 150 molecules functioning as HDAC inhibitors. How can these molecules be classified according to their chemical structures? Are there any software available for this particular...
16 September 2024 7,794 0 View
I need help with suggestions for a thesis title as a requirement for me to achieve a bachelor's degree in engineering
13 September 2024 5,976 3 View
Solar cell simulation
12 September 2024 8,559 1 View
Collateral coronary blood flow is a salient innate compensatory mechanism to the acute or chronic myocardial ischemia. The latter is divided into angiographically and non-angiography visible...
11 September 2024 2,008 6 View
if a material has small crystallite size compared to the other, can we say it is more crystalline than the other?
07 September 2024 4,499 2 View
When I did my experiments, I found that phosphoric acid activated metakaolin could not be cured at room temperature, and it usually took three days for the initial set to occur.
02 September 2024 8,613 1 View
the ancient world. It aims to understand how these precious metals influenced trade, wealth distribution, and power dynamics among ancient civilizations. The question seeks to uncover the ways in...
01 September 2024 9,754 0 View
Assessment Topic and Intro: What do we do when those mandated to protect us are serving other interests than public safety? In South Africa, traffic police corruption leaves road users exposed...
27 August 2024 9,349 1 View
Hello. I need to evaluate the nusselt number for Nu=4.36 for constant heat flux case. My hand calculations give the Nu number at the exit gives Nu=4.36 but I'd like to evaluate properties like,...
13 August 2024 3,149 3 View
I'm new to researchgate and have seen some works people have published on this website. I want you to guide me on the above question thanks
13 August 2024 3,376 2 View
I really need some assistance with Herzberg's theory. I don't see he makes any distinction between job satisfaction and motivation. His list of 'motivators' help employees achieve 'job...
12 August 2024 8,603 2 View