Clinical Chemistry

Clinical chemistry (chemical pathology and clinical biochemistry) is the area of clinical pathology that is generally concerned with analysis of... | Contact experts in Clinical Chemistry to get answers

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Questions related to Clinical Chemistry

Has anyone used demeditec cortisol ELISA assay kit for human specimens?

08 April 2015 9,094 3 View

Is anyone familiar with eclia/elisa vs Radioimmunoassay of plasma renin activity comparative studies?

anyone knows of comparative studies of elisa/eclia methods vs radioimmunoassay of plasma renin activity?

04 April 2015 518 1 View

Is anyone aware of studies or work that has been done on looking at using an Androstenedione based free androgen index?

In our institution, testosterone and androstenedione are measured simultaneously using LC-MS/MS and I have come across patients with clinical signs of androgen excess who have normal testosterone...

04 April 2015 7,095 2 View

Has anyone used demeditec cortisol ELISA assay kit for human specimens?

04 April 2015 3,177 3 View

Is anyone aware of studies or work that has been done on looking at using an Androstenedione based free androgen index?

In our institution, testosterone and androstenedione are measured simultaneously using LC-MS/MS and I have come across patients with clinical signs of androgen excess who have normal testosterone...

01 April 2015 4,031 2 View

After muscle or muscle-skeletal trauma, how long does myoglobin remain in the bloodstream?

After muscle or muscle-skeletal trauma, about how much time clinical analysis can be performed to detect blood myoglobin. What levels are considered positive, and what factors can cause a...

23 March 2015 2,595 3 View

What is the most economic method of determining plasma adiponectin levels when you have up to 1000 individuals?

If kits would be used for determining the adiponectin levels, kindly provide details of how I can get the kits.

16 March 2015 9,948 8 View

Can you please suggest any laboratory methods to make conjugates of indomethacin with amino acids?

We are working on conjunction of indomethacin with amino acids and experiencing certain difficulties. Please share any suggestions you have on lab methods, choice of solvent and confirmation of...

04 March 2015 2,200 1 View

After muscle or muscle-skeletal trauma, how long does myoglobin remain in the bloodstream?

After muscle or muscle-skeletal trauma, about how much time clinical analysis can be performed to detect blood myoglobin. What levels are considered positive, and what factors can cause a...

03 March 2015 9,279 4 View

Can you please suggest any laboratory methods to make conjugates of indomethacin with amino acids?

We are working on conjunction of indomethacin with amino acids and experiencing certain difficulties. Please share any suggestions you have on lab methods, choice of solvent and confirmation of...

03 March 2015 2,071 1 View

What is the most economic method of determining plasma adiponectin levels when you have up to 1000 individuals?

If kits would be used for determining the adiponectin levels, kindly provide details of how I can get the kits.

03 March 2015 948 7 View

Do you have any good protocol for a modified Nelson-Somogyi?

I am trying to develop a method for the analysis of reducing sugars content in biomass samples in microtitre scale. In particular I am using a modified Nelson-Somogyi method (modified because I am...

02 February 2015 8,998 1 View

Can I use freeze dryer to remove DMSO?

I want to use an IP3R inhibitor (xerospongin C) in an in vitro calcium release assay with CHO-K1 cells. I accidentally dissolved the small amount we received in DMSO at a concentration which means...

02 February 2015 10,136 10 View

Has anyone found that very lipaemic serum samples have high haemolysis indices?

On our high throughput hospital clinical chemistry analysers we measure haemolysis, icterus and lipaemia using simple spectrophotometric indices. This is to avoid analysing tests where these...

01 January 2015 5,711 7 View

Does levamisole react with MBTFA?

I am derivatising analytes in a method with MBTFA (at 70 deg for 20 min.) and when I do so my levamisole peak disappears from my trace. From experiments with other derivatisation reagents...

01 January 2015 7,945 3 View

Where can I find the CMS fee schedule for histochemical and cytochemical laboratory tests?

US IVD

01 January 2015 1,260 0 View

Can I use buffycoat directly for pcr which is collected in Li heparin tube?

I just want to know if it works or not and the effect of Li heparin on pcr.

30 December 2014 1,475 4 View

Can I use buffycoat directly for pcr which is collected in Li heparin tube?

I just want to know if it works or not and the effect of Li heparin on pcr.

12 December 2014 1,686 5 View

Can anyone recommend a precipitation buffer or a short centrifugation method of some sort to isolate HDL from citrate plasma?

We need to isolate HDL from citrate plasma, not to quantify HDL cholesterol but to use the HDL fraction for mass spectrometry later on (to measure S1P). I have done extensive research on...

12 December 2014 9,851 5 View

Can anyone recommend a precipitation buffer or a short centrifugation method of some sort to isolate HDL from citrate plasma?

We need to isolate HDL from citrate plasma, not to quantify HDL cholesterol but to use the HDL fraction for mass spectrometry later on (to measure S1P). I have done extensive research on...

08 December 2014 1,939 4 View

Please help me. Which concentration of extract (as a drug) should I use for the detection of DNA fragmentation? What's the approximate concentration?

My extract is ic50 is 0.263 mg/ml.

10 October 2014 7,595 1 View

Is singlet oxygen the mediator of ozone-induced inactivation of F13?

Neutrophils generate large amounts of singlet oxygen (via NADPH-oxidase/myeloperoxidase mediated generation of chloramines). Physiologic activation of neutrophils results in an anti-thrombotic...

10 October 2014 3,227 3 View

Can the power of singlet oxygen releasers be hampered by preanalytic redox reactions?

Just before starting the experiment, should I check if the singlet oxygen release power of the oxidants is still ok? Oxidation: 4 mol/L KI.

10 October 2014 7,375 3 View

Does sw742 cell line has wild type or mutant p53?

Any references on this would be gratefully appreciated.

10 October 2014 7,974 0 View

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