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Hello, I recently bought a pre-engineered e.coli Bl21 containing a pje202 plasmid. Yet I have to grow it on lb-ampicillin-agar to prevent plasmid loss, but I don't want to. I wondered, if there is...
06 June 2018 7,415 1 View
Hello, I want to do some research on bioluminescent pVIB/pje202 e.coli. I am curious about how bright it glows compared to a “normal“ photobacterium phosphoreum strain. Any experiences?? I want to...
06 June 2018 2,487 1 View
Hello, I want to isolate the brightest bioluminescent mutants of photobacterium phosphoreum from my culture, but all colonies look equally as bright. Any ideas, how I could isolate brighter mutants??
06 June 2018 6,953 5 View
Isn't it pure psychoticism to have the most fundamental unit of analysis of a presumed foundational behavior pattern of AN organism INCLUDE MORE THAN ONE ORGANISM'S BEHAVIOR necessarily (or really...
06 June 2018 3,088 3 View
Hello, I recently bought a genetically modified e.coli (pje202/pVIB plasmid), which is bioluminescent. I don't want to grow the e.coli on antibiotic agar forever, because the antibiotic selection...
06 June 2018 6,592 6 View
Hello, I wondered, if there is a possibility to prepare Amp trihydrat from sodium salt without toxic chemicals. I need trihydrate because with this I can make a stable colloid...
06 June 2018 5,261 1 View
Does anybody have any direct experience of this or know of any studies which have compared to two targeting methods of PCRs?
06 June 2018 460 3 View
Survey link: https://goo.gl/forms/mg6HricpW8GChcV63 Based on the results of this survey, our team will develop new ways for scientists to share protocol experience.
06 June 2018 3,836 6 View
There is no conclusive evidence that time is quantized. However, the recent difficulties with the Standard Model, as it nears 50 years, for example, with non-inclusion of gravity in a testable...
06 June 2018 7,877 33 View
I recently bought pVIB plasmid modified e.coli (BL21 strain) and now I want to grow them on LB agar containing ampicillin for selective pressure. I wondered, whether there is a chance due to...
06 June 2018 4,616 4 View
This was sparked by a quote I read ' The way that scientific publishing fetishizes novelty is gross. That's the business model of tabloids, not scientific journals. ' -Ed young
06 June 2018 2,971 6 View
In biochemistry, a metabolic pathway is a linked series of chemical reactions occurring within a cell. The reactants, products, and intermediates of an enzymatic reaction are known as metabolites,...
31 May 2018 5,839 4 View
Can anyone suggest an academic manuscript editing services on "biology education/science education"? Thank you very much for your answers.
28 May 2018 358 9 View
I'm also wondering how you differentiate the two concepts.
14 May 2018 5,853 88 View
I, of course, say YES. But, also, there is a question of when (where/how, as well) that a containing system needs to be discovered. In the case of the more intangible psychology (like much of...
05 May 2018 9,031 0 View
Have wrestled with Bayesian models in R a bit but would like to gain proficiency. Biology/ecology slant would be ideal.
05 May 2018 7,430 3 View
I am interested in any paradox so long as it is explained well. I will be particularly interested in paradoxes in Physiology and Medicine? are there...
05 May 2018 8,000 3 View
How and by what mechanism does the non-living matter assemble into a conglomeration we call life?
05 May 2018 8,081 0 View
AI seems to be pretty popular these days and there are many applications in our daily life. But are there any AI designed to help our researchers to solve any biology questions?
05 May 2018 4,225 3 View
No. Philosophers should not be concerned about what could be without, at some real and abiding "level" (way), being MORE than concerned with what IS -- they should (actually: MUST) discover * the...
05 May 2018 8,775 2 View
Biology
05 May 2018 8,533 1 View
For those of you who teach an introductory biology course spanning genetic through ecosystems biology, what is your preferred lab manual, and why? Thank you, Cynthia
05 May 2018 8,388 0 View
05 May 2018 8,820 8 View
05 May 2018 1,433 5 View