Hello,
I want to do some research on bioluminescent pVIB/pje202 e.coli.
I am curious about how bright it glows compared to a “normal“ photobacterium phosphoreum strain.
Any experiences??
I want to use the e.coli instead of my P. phosphoreum, because it can use way less complex carbon sources.
Compared to e.coli (at least k12), photobacteriad are very sophisticated.
Dear M. Leonhardt,
The bioluminescent process is regulated by the bacterial metabolism and their stage of growth. The physicochemical composition of the growing media also modified the amount of emitted light over time. The emitted light decreases when the bacteria are under suboptimal conditions of temperature, nutrition, aeration, etc., and sometime inducers are also required.
Follow this link to see how much light can be emitted by E. coli (http://jb.asm.org/content/164/1/45.full.pdf) under various conditions. The maximal luminescence was 10 ^12 quanta/s/ml.
In the study of D. G. Deryabin et al. (2016; https://doi.org/10.1134/S00262), they compared Photobacterium phosphoreum (B17-677F), Escherichia coli (E. coli K12 TG1 (pF1) with a lux-CDABEcassette of the genes from V. fischeri), Escherichia coli (E. coli K12 MG1655 (pXen7) transformed with a рUС18 hybrid plasmid bearing an EcoRI DNA fragment containing the structural bioluminescence genes of Photorhabdus luminescens ZM1) and Bacillus subtilis strains (B. subtilis 168 using the P. luminescens luxCDABE genes). The relative light unit was 10 times higher with Photobacterium phosphoreum.
So, the relative luminescent unit measurement will depend of your material and methods.
Kind regards,
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