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I am helping to find manuscripts for an edited collection of papers/chapters on lobster biology, behavior, and management. The scope of this work is all marine crustaceans considered to be...
07 July 2018 8,354 0 View
Mathematical models in science usually have free parameters that should ideally be estimated from empirical data. Sometimes, however, model parameters cannot be estimated because pertinent...
07 July 2018 9,863 3 View
While a new theory with its Memories' capacities/natures/capabilities, _AND_ WITH realist innate guidance aspects coming into behavior patterns throughout ontogeny, may well be easiest to find and...
07 July 2018 2,344 0 View
Greetings, Recently, I've come across a paper which experimenting on the antibacterial test using disc diffusion. An interesting thing I stumble upon is the result were categorized according to 3...
07 July 2018 9,083 4 View
I am trying to measure stiffness of red blood cells in RPMI using AFM force spectroscopy method. In literature, the typical indentation of RBC at a force of 500pN is 500nm. But in my case I see...
07 July 2018 9,076 6 View
I am imaging nucleosomes using AFM. I purchased nuclesomes from EpiCypher. The storage buffer contains 10mM TrisCl, 1mM EDTA, 25mM NaCl, 2mM DTT, pH 7.5. I have the stock solution (2ug/ul) kept at...
07 July 2018 7,392 3 View
While I was teaching a high school biology student, she asked a simple question on diffusion and then I had to tell her about Brownian motion etc, following which we ultimately reached electrons!...
07 July 2018 1,085 1 View
What's the best way to measure stomatal conductance in grasses?
07 July 2018 8,804 3 View
Hello, I just inoculated a new E.coli culture (k12 Gal+ strain) and I recognized something I can't explain, the bacteria lawn (by excessive water) has a branchy appearance, any explanations for...
07 July 2018 3,937 6 View
Hello, I got a question concerning the double layer agar method for plaque assay: 1) Do I really have to use two agar layers, wouldn't it be enough to use one pre-inoculated soft-nutrient agar...
07 July 2018 9,270 5 View
In view of modern science, I realize that Adam could not have been the first human being. But was there still a historical Adam who served as the first theologically significant human being (i.e.,...
07 July 2018 8,969 94 View
Hello, I want to create a self sustaining bacteria lamp using multiple microorganisms that live in symbiosis together. I heavily refer this project to the “biobulb“. My plan is using...
17 June 2018 2,563 7 View
Hello, I recently bought a genetically modified e.coli (pje202/pVIB plasmid), which is bioluminescent. I don't want to grow the e.coli on antibiotic agar forever, because the antibiotic selection...
13 June 2018 6,651 5 View
I recently bought pVIB plasmid modified e.coli (BL21 strain) and now I want to grow them on LB agar containing ampicillin for selective pressure. I wondered, whether there is a chance due to...
10 June 2018 6,312 3 View
I am trying to find paper: Rahmani, A.R., Khan, S.M. 1980. Histology of the olfactory epithelium and the accessory nasal sacs of an anabantoid fish, Anabas testudineus (Bloch). Archives de...
06 June 2018 3,943 1 View
Hi averyone I want write a research but I don’t know how can find the data from internet.
06 June 2018 7,455 4 View
Biology
06 June 2018 7,939 0 View
06 June 2018 9,928 8 View
All, We are trying to determine SOD activity in mitochondria isolated from rat, mouse, and human kidneys. As suggested at the bottom of page 8 of the Cayman instruction manual we read both the...
06 June 2018 6,014 16 View
Clearly. Yes. AND, this is because of baseless, unjustified, unproven and likely false core/"foundational" pseudo-'assumptions' which unreasonably constrain possibilities for explanation (and...
06 June 2018 9,652 0 View
Hello, I recently bought a pre-engineered e.coli Bl21 containing a pje202 plasmid. Yet I have to grow it on lb-ampicillin-agar to prevent plasmid loss, but I don't want to. I wondered, if there is...
06 June 2018 7,355 1 View
Hello, I want to do some research on bioluminescent pVIB/pje202 e.coli. I am curious about how bright it glows compared to a “normal“ photobacterium phosphoreum strain. Any experiences?? I want to...
06 June 2018 2,444 1 View
Hello, I want to isolate the brightest bioluminescent mutants of photobacterium phosphoreum from my culture, but all colonies look equally as bright. Any ideas, how I could isolate brighter mutants??
06 June 2018 6,901 5 View
Isn't it pure psychoticism to have the most fundamental unit of analysis of a presumed foundational behavior pattern of AN organism INCLUDE MORE THAN ONE ORGANISM'S BEHAVIOR necessarily (or really...
06 June 2018 3,036 3 View