In what form do plant sterols exist in the bloodstream? In lipoprotein particles or in extracellular vesicles?
https://en.wikipedia.org/wiki/Viscosity
21 July 2024 3,089 4 View
I've been looking at digsilent to see how HVDC affects the control of generators. It's a simple HVDC connection of buses. The static generator is set up as a reference machine, but as you can see...
13 March 2024 4,582 1 View
I need to calculation the single point of a species, and I submitted a series of tasks, most of them worked, but a few of them failed. and the error code was like: (Everything was normal until...
24 February 2024 1,081 1 View
In my experiment set up, I build a 2 DoF vibration system using springs and low friction slides. I've attached the force sensor on the vibration system and then link it with the cylinder, then...
19 September 2023 2,982 0 View
To prepare the solution of SPION at a specific concentration, I need to dry the SPION after precipitating them by ethanol. However, I always failed to get the powder form but kind of gel. I wonder...
23 July 2023 9,414 0 View
Except adding a small amount of oxygen or increasing the pressure of the growth atmosphere, are there any other methods to inhibit the volatilization of Ga to maintain stable crystal growth...
07 March 2023 9,013 2 View
Hello, I have been performing 9 well experiments over this past week and have had no success in running it. The purpose is to amplify my DNA. In the attached file, all the graphs from the...
28 September 2022 1,386 0 View
I am using the 5 mL Hi-Trap Q FF column to separate my protein/peptide sample. I got the correct responding of UV readings and conductivity before I connected the column to the FPLC unit, but as...
13 November 2021 5,353 2 View
I passaged my hiPSC into vitronetcin coatead plate with ROCK inhibitor in E8 medium. The 2nd day it looks good with about 40% confluency. But after I changed the medium to E8 without ROCK. I...
08 April 2021 7,548 4 View
In traditional industrial manufacturing, what are the problems in the packaging design and application of parts? Can it be solved by smart devices?
25 March 2021 9,159 3 View
Dear sir/Madam, I am Parinaz Parsa the first writer of article "Optimisation of probiotic yoghurt production enriched with phytosterols" and understand that one person is mentioned as writer which...
14 July 2024 9,579 2 View
Is it appropriate to place the ATG codon in front of the gene of interest, since there is a secretion signal that has its own ATG in front of this gene? I need my protein of interest to be...
10 July 2024 3,050 5 View
Hello to everyone, I'm trying to extrtact total RNA from sEVs. I made a first experiment starting from 2x10^10 sEVs quantified by NTA but the RNA obtained was very low (under 20ng/ml) Do you have...
18 June 2024 2,799 4 View
Hi, I am doing western blotting of isolated extracellular vesicles, and I have albumin and calnexin contamination that I don't know why. Is there any way to avoid it?
16 June 2024 9,907 4 View
Please give some insight on this
06 June 2024 2,016 2 View
Ultracentrifugation is the most widely used method for isolating extracellular vesicles (EVs), despite the significant structural damage that EVs suffer during the process.
16 May 2024 9,432 1 View
In the younger boy there is no ejaculate because the prostate and seminal vesicles are not yet functionally developed, and in the female those glands are rudimentary and never develop. (Alfred Kinsey)
29 April 2024 6,483 0 View
I tried to inhibit extracellular microvesicles(MVs) secreted from cells, through using Rock inhibitor(Y27632), calpeptin and several siRNAs(siCDC42, siTSG101, siARF1), but failed to decrease MVs....
23 April 2024 7,369 0 View
Hello, I need to determine tocopheroels and phytosterols by GC-MS but I do not have the reagents to derivatize (TMS, TMCS, BSTFA, etc.) that most of the consulted literature generally uses. I...
22 April 2024 8,178 6 View
Dear Colleagues, We plan to isolate and characterise extracellular vesicles released by dying cells during radiotherapy of head and neck squamous cell carcinoma. Given the duration of...
14 April 2024 494 3 View