Droplet PCR in plastic chips always result an unexpected band, any suggestion of possible contaminations and how to improve?

I am doing droplet based digital PCR inside plastic chips. I can get the correct band, but I also consistently get an unexpected band, slightly shorter. I attached a typical gel image here....

18 February 2021 6,618 2 View

How to interpret this LC3 immunoblotting result for the autophagic function?

I performed an immunoblotting with LC3 antibody (please see the image) upon siRNA-mediated knockdown of two different proteins, A and/or B, with or without Bafilomycin A1 treatment. I would like...

20 October 2019 8,517 4 View

How to make soil suspension?

Hi all, I plan to test effects of some chemicals on soil microorganisms. Culture medium is still hard to finish that. Because they are difficult to mix with soil thoroughly, I hope to make...

20 June 2019 2,941 2 View

Laser capture microdissection-- tissue just not picked up?

Anyone had experience in Laser capture micro dissection of H&E stained FFPE sections mounted on regular glass? I found that the cutting is OK just the tissues won't be picked up. Only leaving...

11 March 2019 7,137 6 View

How can i proof that the doped atoms in one lattice host occurs alignment?

21 October 2018 8,694 5 View

Is it possible to make emulsion about hundred micrometer in diameter with molecular surfactant?

I am trying to compare nanoparticles and molecular surfactants for hydrate formation process. Pickering emulsion stabled by nanoparticles are usually in hundred micrometer in diameter. So I am...

21 July 2018 7,009 3 View

What is the chemistry related to darkening of CH3NH3PbI3, HC(NH2)2PbI3 precursor solutions, after aging?

29 March 2017 8,288 3 View

What is the highest efficiency of hole-conductor-free perovskite solar cell?

What is the highest efficiency of hole-conductor-free perovskite solar cell? Thanks.

19 January 2017 2,056 3 View

Do lifetimes extrapolated from time-resolved photoluminescence decays and transient absorption decays reflect the same carrier processes?

23 October 2016 196 2 View

How to choose a suitable E2 when performing in vitro Ubiquitination assay?

22 August 2016 327 4 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Hi..how to isolate crude HDL (all subfractions) from human serum by size exclusion chromatography?

I would like to know about isolation of crude HDL from serum by SEC. Is it possible to perform with a serum sample volume of

19 February 2021 5,576 2 View

Is it possible to remove the cells from a tissue and keep only the extracellular matrix for proteomics?

Hello! I would like to know if there is a way to flush/remove all the cells out of a tissue and keep only the extracellular matrix, in order to analyse its composition using e.g. proteomics....

14 February 2021 1,602 4 View

Any model suggestion for the Electronic Health Records dataset ?

I have sequentially measured the time-series Electronic Health Record patient dataset. This is 7 years of research and each patient's blood pressure, Hba1c, LDL, Hb, Baseline Age,...were measured...

09 February 2021 4,933 8 View

Meniscus formation matrigel?

Hey guys, I am currently doing tube formation assay on matrigel coated coverslips, and i wonder if there is any possibility to get rid of the meniscus formation? I have only a small part of the...

09 February 2021 6,979 3 View

Scanning Electron Microscopy for Extracellular Vesicles?

Can anyone provide or recommend a detailed protocol for preparing extracellular vesicles for SEM? Most protocols that I have found that list the necessary reagents and individual steps are for...

02 February 2021 4,475 3 View

Which ABs concentration for EVs immuno-staining?

Hi all, I have to do a phenotyping study on cell culture derived EVs, in particular I should stain them using primary labelled ABs anti-EVs markers before proceeding to analyze them through...

25 November 2020 4,601 1 View

Can someone please share how to add a spherical wrap / correction to a visual stimulus presented to mice using python?

Hi all I use psychopy 3 to show mice a visual stimulus on an LCD screen while preforming acute extracellular recordings in vivo. I understand that one should use a spherical wrap to the stimulus...

22 November 2020 7,905 2 View

Recommend equipment of in vivo recording in mice brain?

Hello, I am trying to set up an in vivo recording system in mice brain. Which company do you recommend? Neuralynx? TDT? NeuroNexus? Which one do you guys use and why? Please let me know. Thank you!

12 November 2020 2,508 1 View

What is the effect of sulfa drugs on lipid profile.. And what is the mechanism of action on them?

Sulfa drug increase the the cholesterol and low density lipoprotein.. What is the mechanism of action of this drug which increase these types of lipid...

06 November 2020 4,051 3 View