Why am i not getting solid after reacting APTES with salicylaldehyde?

The reaction of APTES with salicylaldehyde in ethanol leads to the formation of imine compound yellow solid which is mention in literature, but I am not getting yellow solid I don't know why? when...

28 February 2021 2,955 2 View

Why gel electrophoresis is taking too much time to run ?

I am working with TBE buffer and prepared 1% agarose gel. Previously it was taking around one hour to run but now two and half hours. I have increased the voltage from 90 to 110 but this also helped.

08 December 2020 7,416 5 View

What are the recent research topics in field of Soft Robotics based on AI and ML?

I am working in the field of practical robotics since last five years and got acquainted with AI and ML recently. I know Python and learning AI and ML using python from different sources. I want...

24 October 2020 793 6 View

Can Solar Energy take part in fighting against COVID-19?

Can "Solar-UV-cellular-damage" be an effective method to disinfect water bodies against COVID-19 in countries with a hot climate?

10 May 2020 2,556 12 View

While performing energy minimization step in GROMACS I am getteing following error. Can anyone help with this minimization problem?

gmx mdrun -v -deffnm em Back Off! I just backed up em.log to ./em.log. Running on 1 node with total 8 cores, 16 logical cores Hardware detected: CPU info: Vendor: GenuineIntel Brand:...

11 March 2020 2,902 1 View

What is iodine number in terms of activated carbon? What are the effect of iodine number on activate carbon porosity ?

11 March 2020 5,239 3 View

How to convert Netcdf file to ASCII data file?

I want to convert NetCDF file containing MODIS LAI data into ASCII file but not getting enough help from google search. if anybody is familiar with this, please give some inputs.. I am using R and...

18 February 2020 6,100 5 View

HI, Can I engineer any immortal human T cell line with the murine TCR of Ovalbumin ? Just like OT1 mice T cell have the same receptor ?

I am planning to develop a Human T cell line having the potential to kill OVA expressing tumor lines. Mostly we have to take out Tcells from OT1 mice which is expensive in overall cost. If I...

25 December 2019 3,417 2 View

What is the best suitable High Voltage amplifier to excite PZT ceramic C-91 patch (fuji ceramics ltd.)?

I need some information regarding a suggestion for piezo linear amplifier/High voltage amplifier suitable to operate at a peak voltage of 500V/mm for piezo ceramic patches (Rectangular C-91)....

27 November 2019 8,466 8 View

How we encapsulate vitamin-D with chitosan nanoparticles and how much conc. of ethanol we use to dissolve vitamin-D ?

We are trying to encapsulate vitamin-D in chitosan. Kindly suggest me the solubility of vitamin-D in ethanol and the simplest method to encapsulate vitamin-D. Thanks with regards

05 November 2019 9,341 1 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What are the most relevant MSc proposal topics for Petroleum Production Engineering at the moment?

I am a Petroleum Production Engg MSc student at Robert Gordon University in Aberdeen and I am in the process of selecting a topic for my MSc Engineering Investigation proposal. I would very much...

01 March 2021 7,396 4 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Antibody anti-IgM Bovin conjugated Biotin ?

Hi everybody, Can anyone please suggest me an antibody anti-IgM Bovin conjugated Biotin ? I would like to use it in LUMINEX with a streptavidin conjugated phycoerythrin reporter. Thanks in...

25 February 2021 9,659 2 View