How to remove the endotoxin from polysaccharides or oligosaccharides?

More Sudhansu Sekhar Choudhury's questions See All

How should I measure the DLS of my protein samples?

I have been trying to measure the size of the human lysozyme protein with DLS. The instrument is Otsuka ELS-Z 1000. The upper and lower measurement size limits are 10 nm and 4000 nm respectively,...

18 February 2024 9,885 3 View

Does biometrics lower security since biometrics recognition is probabilistic?

The recognition process of all types of biometrics relies on the probabilistic judgment of variable physical and / or behavioral features of human beings. However, an authentication system...

16 February 2024 3,773 5 View

Does the ibrav change when we use vacuum for an atomic system in a quantum espresso input file using nanolabo?

Normally for ZnO, ibrav = 4 (i.e hexagonal). Now I want to perform gas sensing studies. So I create a vacuum of 10A in nanolabo where the atoms are placed. The ibrav now changes to 12 (i.e...

12 September 2023 3,180 0 View

How to calculate mixing enthalpy of any HEA alloy?

If molar fraction, atomic radius and electro negativity values are known then how to calculate mixing enthalpy?

06 August 2023 4,945 2 View

How to vary elastic modulus with shear strain in ABAQUS?

Is there any way to vary elastic modulus with shear strain using field variable in ABAQUS?

21 April 2023 7,572 2 View

Fatigue of weld composites?

I want to understand the mechanism of fatigue failure of weld composites when the force applied is parallel to the welding direction. Can anyone point out any research article?

11 August 2022 3,388 5 View

Hello! I have SAED pattern of my metal nanoclusters. How do I interpret it?

It is the SAED pattern imaged through JEOL TEM.

04 August 2022 6,668 2 View

What is the protocol for conducting a multiplex PCR?

Curious to know the method

10 June 2022 4,287 2 View

What is the percentage of cells with Barr body present in female buccal mucosa, in general?

As we have observed from a practical session that, not all buccal squamous epithelium cells are having Barr body in them. My query is what is the percentage of cells with Barr body could be...

10 June 2022 976 2 View

Can anyone tell me how to stimulate oral epithelial cells for cytokine release ?

I am trying to stimulate oral epithelial cells for cytokine release. I have tried different endotoxins, but not able to get any results. Any suggestions?

10 December 2021 1,693 2 View

Conjugation of PEG-Amine to an Amino Acid Using EDC?

I am attempting to conjugate PEG to an amino acid at the C-terminus, for the purposes of producing nanoparticles. I have been told that PEG modified with amine groups can be used for this purpose,...

31 July 2024 2,033 1 View

Can anyone guide me the best method to analyze the XRD data???

I will be doing an XRD on a sample which is powdered in natured and is a hydrogenated compound. I would like to know how we can understand the individual components and components by using the...

28 July 2024 4,548 2 View

EDC carbodiimide and NHS easter 's half life among different pH?

It has been long known that EDC carbodiimide and NHS easter's half life agains hydrolysis are highly related to pH. However, very surprisingly, not yet found a paper giving a table/chart of their...

25 July 2024 8,738 0 View

What does the difference \lambda_j - \lambda_{j+1} represent in relation to the conjugate partition of \lambda?

For a partition of an integer \lambda, what does the difference \lambda_j - \lambda_{j+1} represent in relation to the conjugate partition, , with \lambda_j being the j-th element of \lambda?

23 July 2024 8,008 1 View

Is it possible to conjugate an NHS ester to the N-terminus of a peptide ON RESIN?

So i have a few peptides (synthesized by SPPS and not cleaved from resin) and I want to add a maleimide label. We have NHS-PEG-Maleimide and from literature it is evident that the free amine group...

18 July 2024 4,075 3 View

Does anybody have Anti-Claudin8 and Anti-Claudin2 Primary conjugated Ab (Target Species: Human)?

I need Anti-Claudin8 Primary and Anti-Claudin2 conjugated Ab for my immunofluorescence experiment. If anybody has worked on it. Please share the accession number/details.

14 July 2024 485 0 View

Would it be possible to determine how many Cys I have based on Ellman's assay?

I want to predict the DAR ratio based on the number of cys available for conjugation to Cys residue. I use Ellman's assay to predict the concentration of Cys after TCEP reduction. I gain a...

02 July 2024 612 6 View

Conjugate Pad absorption suddenly changed?

Hello, I am doing some testing on lateral flow devices/materials and have run into a slight issue where I do not understand what the cause may be, but I have some ideas. General Experimental...

01 July 2024 7,960 3 View

What are the types of Raney Nickel?

I'm interested in Raney Nickel and its composition. While it's usually a Nickel-Aluminum (Ni-Al) alloy, I recently saw an article mentioning Ni-Zn and Ni-Sn as Raney Nickel too. #RaneyNickel...

11 June 2024 355 2 View

What is best stabilizing buffer for conjogation of quantum dots to antibodies?

We are researching the conjugation of various antibodies to quantum dot microspheres and europium microspheres. The sustainability results have been unsatisfactory. Which stabilizing buffers do...

25 May 2024 7,663 3 View

Aly Savadogo

Dear Sekhar

I recommend you to read this paper

https://sites.ualberta.ca/~csps/JPPS10_3/MS_996/MS_996.html

Sudhansu Sekhar Choudhury

than q Aly, i ready gone through that paper and tried with triton x-114 but its not showing that much effective working, apart from that any other chemical tretment is there , pls share with me