Can anybody suggest me how to do E-rosette inhibitionand lymphocytotoxicity assay in antithymocyte immunoglobulin (ATG) in a descriptive manner?

Sudhansu Sekhar Choudhury @Sudhansu-Choudhury

01 June 2018 0 8K Report

Hello

I purified a specific antibodies (polyclonal) named as antithymocyte immunoglobulin (ATG) from immunized horse plasma by affinity chromatography, now i need to do certain potency test in the collected antibodies like E-rosette inhibition assay and lymphocytotoxic assay, please suggest me a good and standardised protocol for the above test

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