I isolated the thymocytes from human thymus and preserved in Liquid nitrogen by using 10% DMSO, further I need to coat this collected cells in a microtter 96 well plate for doing ELISa method manually, can any body suggest me how to coat those cells in the plate? is it necessary to remove the DMSO from the cell suspension before coating the cells in the plate? how many number of cells we need to use per well for coating purpose? what kind of buffer or media need to prepared for cells to coat? how many hour I need to keep for proper coating?