I am trying to do primary culture from an old mouse (about 2-year-old) for some reason.

Usually, I tried to culture myofibroblasts and dermal fibroblasts but failed continuously.

The cells should last at least for passage 4 or 5, but whenever the passage increases,

the cells deform and differentiate, not dividing anymore.

I have tried several growth factors and media but didn't work.

Is it possible to do primary culture from the old mouse?

Thank you.

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