I am trying to do primary culture from an old mouse (about 2-year-old) for some reason.
Usually, I tried to culture myofibroblasts and dermal fibroblasts but failed continuously.
The cells should last at least for passage 4 or 5, but whenever the passage increases,
the cells deform and differentiate, not dividing anymore.
I have tried several growth factors and media but didn't work.
Is it possible to do primary culture from the old mouse?
Thank you.