25 Questions 61 Answers 0 Followers
Questions related from Satyajeet Salunkhe
I am not clear what is rule behind mirna and mRNA targeting pair,because each mirna target officially 100s of target. In such cases how one will validate legitimate mirna mRNA target. Assays...
04 April 2019 7,916 5 View
I want to know how to design primer for primary miRNA QRT PCR
03 March 2018 309 7 View
I use trizol for RNA isolation,but we store phenol choroform phase -30 , I want to know is it good to do isolation of protein later after storage -30 of the above mentioned phase or it may affect...
11 November 2017 7,505 0 View
I read recently that cholesterol conjugation remvoes need of lipofectamine and enhance efficiency of transfection of mimics.If any one has used please answer
12 December 2016 6,853 1 View
I am currently working with lymphocyte ,I want to know if any one has used the miRNA mimics or inhibitor with primery cells
11 November 2016 3,427 4 View
i am currently making assay of syber green based real time pcr for detecting rare isoforms.To make them specific ,can i use say 1 p mole of forward and 5 p mole of reverse primer,technically for...
07 July 2016 8,347 4 View
There are certain miRNA originated from the intron of genes. I could not found such tabulated form in which genes and respective miRNA are given Can anyone provide the same.
12 December 2015 1,664 3 View
During literature search I found miR 17/92,miR-17 approximately 92, miR 17~92 I am not able to understand practical meaning of these annotations in microRNA. Can anybody help to me to understand...
10 October 2015 6,828 1 View
I am currently working with gene I had problem in that at higher dilution the non specific band is incresing non template control is clean,tried with higher temperatures, SYBER GREEN based real...
10 October 2015 6,240 3 View
RNA seq data analysis by intron centric analysis gives insight about exon skkiping ,I have attached photo of excel file I have. Can anyone help me how to interpretate it eg...
07 July 2015 9,569 4 View
I need to analyse microarray data ,so I need latest algorithms can any body help me to find ..
06 June 2015 7,357 0 View
There are many E3 ligases in genome of mouse so if anyone wants to now that protein under consideration is ubiqutinylated by which E3 ligase, I would like to now is there any rule or in silico...
04 April 2015 4,462 3 View
During replication of DNA scientist shown that stemm cell retain their own copy of DNA and avoid mutation in genome ,but how it can be possible ,and is it possible in other cell type
12 December 2014 3,204 2 View
In my study there was two enzymes structurally very diverse but they have same enzymatic activity. I want to know how we can predict that both have same surface charges or potential or sequence...
09 September 2014 3,104 5 View
it was one of the question asked in class
09 September 2014 7,952 0 View
Transposon mediated mutant help to get genetic insights are there any specialized vectors to do same?
08 August 2014 4,862 3 View
I used expasy for developing homology model I want to know how to check validity?
07 July 2014 3,121 21 View
In my experiment I got false MTT activity in-spite drug was supposed to be cytotoxic I just want to get insight about limitations of MTT.
06 June 2014 2,294 10 View
I have protein that has DNA binding activity. I want to know how I can predict which part of my protein is interacting with DNA by in silico method.
06 June 2014 5,785 1 View
We have MN kit in our lab nucleo spin, but I want to know whether it can be used for isolation of apoptotic DNA.
06 June 2014 4,636 4 View
In my case, hela cells are dying by drug treatment. I want to know if anybody can suggest which marker protein affected?
06 June 2014 6,031 0 View
I just want to know why the buffer contains the NaCl.
05 May 2014 9,312 9 View
I need mouse drosha overexpression cell line ,can any one provide
01 January 1970 9,384 1 View
I want to transfect mirna mimic /inhibitor to primary B cells,Can any one provide the protocol for Nucleofection of mirvana mimic ,inhibitor via nucleofector4 D X
01 January 1970 3,054 1 View
I am currently involved in project that need to transfect B cell with siRNA via NUCLEOFECTION ,problem is viability varies 80 to 16 percent.dose any one faced same problem ,if yes please let me...
01 January 1970 428 1 View
