I think those vectors can be used for the same reason. you need to know the level of activities elements. I hope this answer may help you
Article The pJan25 vector series: An enhancement of the Gateway-comp...
Retrotransposons
Article Retrotransposon-based molecular markers for assessment of ge...
Transposition in genetics have a variety of functions many of which can confer an advantage on the organism. For a nice review, see http://www.annualreviews.org/doi/abs/10.1146/annurev.cb.06.110190.001501?journalCode=cellbio.1
I am not clear what is rule behind mirna and mRNA targeting pair,because each mirna target officially 100s of target. In such cases how one will validate legitimate mirna mRNA target. Assays...
03 April 2019 7,959 5 View
I want to know how to design primer for primary miRNA QRT PCR
02 March 2018 343 7 View
I use trizol for RNA isolation,but we store phenol choroform phase -30 , I want to know is it good to do isolation of protein later after storage -30 of the above mentioned phase or it may affect...
10 November 2017 7,550 0 View
I read recently that cholesterol conjugation remvoes need of lipofectamine and enhance efficiency of transfection of mimics.If any one has used please answer
11 December 2016 6,890 1 View
I am currently working with lymphocyte ,I want to know if any one has used the miRNA mimics or inhibitor with primery cells
10 November 2016 3,458 4 View
i am currently making assay of syber green based real time pcr for detecting rare isoforms.To make them specific ,can i use say 1 p mole of forward and 5 p mole of reverse primer,technically for...
06 July 2016 8,389 4 View
There are certain miRNA originated from the intron of genes. I could not found such tabulated form in which genes and respective miRNA are given Can anyone provide the same.
11 December 2015 1,695 3 View
I am currently working with gene I had problem in that at higher dilution the non specific band is incresing non template control is clean,tried with higher temperatures, SYBER GREEN based real...
09 October 2015 6,288 3 View
During literature search I found miR 17/92,miR-17 approximately 92, miR 17~92 I am not able to understand practical meaning of these annotations in microRNA. Can anybody help to me to understand...
09 October 2015 6,871 1 View
RNA seq data analysis by intron centric analysis gives insight about exon skkiping ,I have attached photo of excel file I have. Can anyone help me how to interpretate it eg...
06 July 2015 9,592 4 View
I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.
11 August 2024 9,101 4 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!
11 August 2024 3,770 4 View
Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...
10 August 2024 7,180 0 View
I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...
10 August 2024 7,429 2 View
How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?
09 August 2024 7,718 0 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...
07 August 2024 1,937 1 View
A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.
07 August 2024 5,307 1 View