I want to detect ROS production in cells but spectrophotometrically, using an UV-visible spectrophotometer. As where I work we don't have a fluorescence reader (as used with the h2DCFDA probe), I wonder if you could recommend which non-fluorescent dyes or reagents can be used for this purpose.
Thanks in advance!
Perhaps what you search is in https://pubs.rsc.org/en/content/articlelanding/2018/ay/c8ay01339j
Gustavo A M Sáfar thanks a lot. That's a very useful and interesting article, and I found that NBT reduction assay is a good, cheap and easy candidate. I wonder if it could be applied in a 96 well plate for cell culture other than immune cell lines.
Santiago, about this particular detail, I can't help you. I am a physicist, not a biologist.
Article Reactive oxygen species generation in aqueous solutions cont...
You may check this article.
- Badges
- Science topic
- Similar topics
- Physical Sciences
- Physical Phenomena
- Luminescence
- Fluorescence