Has anyone tried eBioscience Foxp3 / Transcription Factor Staining Buffer Set with TdTomato+ cells?

Hi all, I stained tdTomato+ cells (PBMCs and Jurkats) with eBioscience Foxp3 / Transcription Factor Staining Buffer Set for the foxp3 and I completely lost the signal of tdTomato. I tried to fix...

10 February 2021 5,863 2 View

How do I correct an inaccurate citation?

I was asked to verify a citation: Drugs and galencials, by D. C. Garratt, B.Sc., Ph.D (Lond.),... The author's first name is Emerson and the last name is Beeler. It was incorrectly cited as...

03 February 2021 5,272 3 View

Is there a way to get rid of TEA in a TLC plate in order to do proper staining?

Dear colleagues: I have been trying to establish a few HP-TLC methods in the lab. In general, for quantification purposes, I prefer CuSO4+acid charring, to primuline staining. Lately I have been...

20 January 2021 9,019 1 View

I have a challenge with a structure I optimized it's weight but the frequency constraint is not satisfied?

I know that frequency depends on material properties, geometry, support conditions and in plane loading. I have played around with these figures except that I maintained the same material steel...

26 December 2020 4,877 9 View

How can I coat reduced graphene oxide on glass substrate?

I dispersed reduced graphene oxide in NMP solution and tried to coat it on a glass substrate using dip coating system. But I am not able to get a uniform coating. Please suggest me a solvent and a...

26 November 2020 8,929 4 View

How can I measure the sheet resistivity of my reduced graphene oxide thin film?

I tried to coat my NMP dispersed rGO sample on the glass substrate by dip coating system. Then I characterized it with four probe system for sheet resistivity measurement. But I got a very high...

16 October 2020 3,696 3 View

Heat and plastic in MTBE lipid extraction?

When extracting lipids with the MTBE method, the indicated temperature is RT. In addition, drying the samples in a speedvac is mandatory due to the presence of water and such procedure is long and...

14 October 2020 9,776 2 View

Does anybody have an idea of how to solubilize hydrophobic peptides?

My hydrophobic peptide (12 AA) is soluble in 100% DMSO. Given that I should encapsulate it in hyaluronic acid nanoparticles, I need that peptide is soluble or partly soluble in water. Does someone...

03 March 2020 8,025 4 View

How do I choose which EEG trials to run in Common Space Pattern (CSP)?

I am currently working on a binary classification of EEG recordings and I came across with CSP. As far as I understand, CSP allows you to choose the best features by maximizing the variance...

20 January 2020 7,536 2 View

For researchers who have a P-97 Micro-pipette puller from Sutter instruments, can you help me get the values of factory pre-programmed Program #1?

We have lost the parameters of this program by mistake in our lab. And we need to reenter those, to our new P-97 puller. A picture would be good. I appreciate the help.

15 October 2019 5,710 2 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Why does two different bioinformatics tools for the same proteins show different subcellular localizations?

During a computational analysis, I found that for a protein of a plant, two different subcellular localizations are seen using CELLO and WoLF PSORT. For example, in CELLO, it identified the...

01 March 2021 6,729 2 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View

Purified protein requires Calcium for activation, but the elution buffer I used seems to interact with Calcium, help?

09 February 2021 6,946 4 View

Why am I getting such large heats of dilution in my Isothermal Titration Calorimetry Experiment?

I am trying to measure binding of calcium to an expressed protein we are studying and am getting very large heats of dilution for the calcium solution in my control tests. We prepared a 1 M CaCl2...

07 February 2021 7,532 1 View

Ac16 cardiomyocytes cell line culturing ?

I have faced a problem with culturing Ac16 cardiomyocytes cell line. Day 0 I plated 200,000 cells/2ml (well) in 6 wells plate in FGM. Day 3 I put my cells on Low serum medium. When I changed...

24 January 2021 1,456 2 View

How can you accurately induce calcium overload and measure membrane potential in cultured Tg2576 neuronal cells using whole cell patch clamp method?

Hi there, I'm currently working on a project where i need to design an experiment but as i have little to no experience with this level of experimental design and no face to face help i am really...

22 January 2021 6,728 3 View

Is there any method I can use to isolate single cells (i.e cardiomyocytes and non-cardiomyocytes) from snap from rat heart tissue?

I have GD18.5 heart tissue currently snap frozen in -80, apart from mRNA and protein analysis on whole tissue, is there a way of isolating single cell populations to study localization of a...

19 January 2021 4,044 3 View

Why calcium oxide nanoparticles becomes black at 650 degree temp?

Please tell me, which temp can I select for neglect black color of the Calcium oxide nanoparticles ..

14 January 2021 5,341 5 View

Does soft algniate hydrogel influence calcium concentration of a solution (cell culture medium)?

05 January 2021 6,066 4 View