We are performing the ACE activity assay in pancreatic tissue homogenate by using fluorescent substrate. For that we are using ACE inhibitor captopril but we are not very confident about its concentration.
PDE value for Tert-Butylamine
24 July 2024 1,166 0 View
Hello, recently i am an issue swith cloning where I am using the subclone method to insert my gene length is 1.5kb and my vector(pMAL-c6T) is 5kb I got the colonies which i screened through the...
04 July 2024 7,977 2 View
Context In the realm of cybersecurity, Transport Layer Security (TLS) is widely used to secure communications over a computer network. Despite its robust encryption mechanisms, TLS is still...
19 June 2024 6,595 3 View
I'm trying to synthesize a highly polar compound which is uv inactive (In TLC charring at the bottom- 0.1RF/20%MeOH:DCM ,MP) . I have confirmed the compound by simple mass and H'NMR spectrums but...
22 May 2024 9,818 3 View
I would like to calculate the gear wheel and gear tooth deformation due to rotational speed? The gear shaft is mounted on hydrodynamic bearings. What are the boundary conditions need to apply?...
21 May 2024 8,067 0 View
Dear In the UNIVERSE from a sand grain to Sun including man, all are bodies of different sizes. Every body has a SHAPE//FORM A body with a shape gets a NAME Every body has a LIMIT And every body...
18 May 2024 3,012 0 View
Please anybody suggest me which ls-dyna material card is suitable for windshield sealant material to prepare material card and to validate for crash performance Thank you in advance.
16 May 2024 2,569 0 View
Hello friends, i am performing bader charge analysis for methylcyclo hexane (MCH)/ceria system using quantum espresso. while performing analysis, the number of electrons generated in ACF.dat is...
12 May 2024 7,264 3 View
I am working on isolating Naive CD4+ T cells from human PBMCs using the Miltenyi AutoMacs. However, I am obtaining a higher percentage of Naive CD4+ T cells from the PBMCs than expected. I would...
25 April 2024 4,658 1 View
more details about ls_dyna material *Mat_252
22 April 2024 5,398 0 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...
06 August 2024 5,801 4 View
During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...
06 August 2024 726 3 View
Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?
29 July 2024 10,023 2 View
Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....
23 July 2024 7,292 3 View
I am new to research. The boiling point of triethyl amine (NEt3) is 89 degrees centigrade. I am skeptical that when I will let the hydrogen escape which is getting generated in situ, I will also...
21 July 2024 7,284 4 View
To establish an orthotopic HCC model, C57BL/6 mice (male, 6-8 weeks) were orthotopically implanted with 3x106 Hepa1-6 cells (Over-expressed with a human gene) in 20uL Matrigel into the left lobe...
18 July 2024 9,177 2 View
when we grow biofilm in test tube, the pipette often do not reach near the content. so we have to decane it directly or use a smaller test tube.
17 July 2024 3,532 0 View
I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...
15 July 2024 6,668 0 View