HEK cell is Clamped at different membrane potentials (-100 mV to +90 mV; 10 mv Step with Vh -60 mV) of GluK2 receptor and recorded the current by application of 10 mM L-Glutamate.
Herewith I attached data excel file having individual data points (mV and pA)
Could you please explain how to plot the rectification index curve by using these data points? Just for the example purpose, I have given the N=1 data points.
Thank you.
Balazs Ordog
Hi Rajesh, take peak current for each clamped potential, then plot these peaks as the function of the clamp potential. Then you will have your current/voltage (I/V) curve.
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