Hi, Can anyone suggest that Lab bench and other settings for current clamp (Action potential) pClamp software. If I am setting the Lab bench Input settings mV for IN=0, the popup showing that "bad membrane signal". Analog IN units must be in Amps with or without a standard SI prefix to be compatible with this test (eg A, Amps, nA,pA the signal Imeb is in 'mV'. How can I set for action potential recordings? Here I attached the settings screenshots ppt slides for best understanding the problem,
Make sure all of your signals are properly configured. Follow the setup instructions for your particular equipment:
http://mdc.custhelp.com/euf/assets/images/KB_962_Set_Up_Clampex.pdf
Are you telegraphing properly? The amp needs to know if you are in voltage clamp or current clamp for it to switch Vm recording to I recording.
What amp are you using?
The error may be because this is for a seal test in voltage clamp and you are in current clamp mode on the amp?
Hi Rajesh,
I am assuming here that you have pClamp 10 and Axopatch 200B as per the question you submitted on LinkedIn. One last bit of useful information is, which Digidata model are you using?
The suggestion of Olivier to telegraph information from the Axopatch to the Digidata and Clampex is very good. I am just not sure that you can telegraph from the Axopatch 200B. That's a question you can ask Molecular Devices directly.
To configure pClamp for current clamp recording with an Axopatch without telegraphing I would use either Clampex or Axoscope software (both parts of pClamp). Historically I have used Axoscope because I didn't telegraph information from the amplifier (you can't receive telegraphed information on Axoscope whereas on Clampex you can) which in my case was also an Axopatch 200B. Then I would proceed as such:
- I am assuming your reference to "IN=0" actually refers to "IN 0" a default signal created by pClamp for the Digitizer Channel "Analog IN #0". So in this case, "IN 0" does not refer to "I=0" which you can find on the Axopatch 200B mode selector. Instead, "IN 0" in pClamp means "Input signal number zero", it's just the name of the signal created for "Analog IN #0" (Analog input number zero) by default in pClamp.
- Anyhow, you may configure this default signal in current clamp, or you can create a new signal ("Add..." button in the "Signals" area of the Lab bench window). When you select the signal to be configured you then choose the unit (in your case most likely mV), the scale factor (I guess this should match the position of the "Output gain" selector on your Axopatch 200B amplifier) and the offset if any.
- Then click OK and your signal should be set in current clamp mode. As confirmation, in your scope window the y axis should show the name of the signal (in this case "IN 0", unless you created a new signal) and the unit in mV.
One potential source of error might be if the scale factor you entered is not accepted by the software for some reason. In this case try using the "Scale Factor Assistant" (still in the Lab Bench window).
I hope it helps.
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