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Questions related from Purva Gupta
I have to check adsorption of ammonia via synthetic matrix. Later on will take it for in-vitro analysis. Many have used a standard of (NH4)2SO4 but have added 2mM ammonium chloride for in-vitro...
08 February 2018 4,889 2 View
I did an experiment for calculating porosity of collagen membrane and it gives 100% porosity. Cells are growing on that scaffold, without passing out from scaffold. So, wanted to know the meaning...
21 November 2016 9,185 5 View
I have plated 0.1 million cells per well in a six wells plate. How many cells should I get per well at maximum after harvesting the plate?
07 March 2016 583 2 View
We retrieved MSCs from bone marrow and cultured in 6 well plate. But everytime whenever we trypsinized the plate, only 1 million cells were obtained from 6 wells, where we put 0.1 million cells...
15 February 2016 7,047 5 View
Is Reductive alkylation (schiff's base) of N terminus-NH2 group of protein and aldehyde( -OH) group possible at PH 7-8? or only acidic PH 4-6?
09 July 2015 6,770 1 View
I have to perform Solid phase purification via Affinity chromatography in which i have to immobilize my polymer (Maleimide PEG Carboxylic acid and Maleimide PEG hydroxyl) to the stationary matrix...
16 April 2015 4,813 3 View
Can anyone suggest a technique for purification of protein (150kDa) and it's PEGylated conjugates (appx. 170 to 200 kDa) other than Automated SEC- superdex 200? In my lab I am unable to separate...
30 March 2015 7,370 6 View
