Purva Gupta

6 Questions 7 Answers 0 Followers

Questions related from Purva Gupta

What is the meaning of 100% porosity of a collagen scaffold?

I did an experiment for calculating porosity of collagen membrane and it gives 100% porosity. Cells are growing on that scaffold, without passing out from scaffold. So, wanted to know the meaning...

21 November 2016 9,173 5 View

Plating Bone Marrow derived Mesenchymal Stem cells in six wells plate

I have plated 0.1 million cells per well in a six wells plate. How many cells should I get per well at maximum after harvesting the plate?

07 March 2016 574 2 View

What is the doubling time of Mesenchymal stem cells ?

We retrieved MSCs from bone marrow and cultured in 6 well plate. But everytime whenever we trypsinized the plate, only 1 million cells were obtained from 6 wells, where we put 0.1 million cells...

15 February 2016 7,043 5 View

Is Reductive alkylation (schiff's base) of N terminus-NH2 group of protein and aldehyde( -OH) group possible at PH 7-8? or only acidic PH 4-6?

09 July 2015 6,759 1 View

Which immobilization (stationary) matrix should I use to immobilize hydroxyl/carboxylic end of Maleimide PEG Hydroxyl/Carboxylic acid?

I have to perform Solid phase purification via Affinity chromatography in which i have to immobilize my polymer (Maleimide PEG Carboxylic acid and Maleimide PEG hydroxyl) to the stationary matrix...

16 April 2015 4,803 3 View

Can anyone suggest a technique for purification of protein and it's PEGylated conjugates other than Automated SEC- superdex 200?

Can anyone suggest a technique for purification of protein (150kDa) and it's PEGylated conjugates (appx. 170 to 200 kDa) other than Automated SEC- superdex 200? In my lab I am unable to separate...

30 March 2015 7,365 6 View