Caspases are known to play a major role in apoptosis pathway. Is there any caspase among the caspase family whose role in apoptosis pathway is not known yet?
any scientific method?
20 January 2024 7,632 0 View
Hi, My research project had numerous results and because the conference paper is usually just 5-6 pages long, I could not discuss all my results in it. However, is it still possible to mention...
04 May 2020 721 5 View
I am trying to run geometry optimization of a catalyst structure and a reactant on its surface. AFter the simulation, I observed 10 atoms (2 Oxygen and 8 hydrogen atoms) from the reactant molecule...
23 April 2020 2,959 0 View
We all know that digestive enzymes speed up the chemical reaction and helps in food digestion. I was wondering if in general it takes 6 h to digest a food with the help of digestive enzymes then...
04 October 2019 5,655 3 View
I recently coated the 96 well plates with 0.1 % w/v (1:10) dilution of Poly-L-Lysine for seeding the HEK-293T cells. However, I found that the attachment of the cells to the surface is not...
24 May 2019 476 2 View
I have to perform fluorescence based assays in 96-well plate using HEK-293 cells. The experimental procedure involves transfection in cells and then treatment with drugs for testing drug efficacy....
01 May 2019 3,738 9 View
Enzymes are synthesised as zymogens and then make a transition to active form when required, is this true for every enzyme or are there any exceptions to that?
17 March 2019 4,480 3 View
I have expressed EGFP-N1 plasmid (1ug) in MCF-7 cells. Upon taking the confocal images under 40x oil I have seen the expression all over the cell? What is the possible reason for that? Can anyone...
27 February 2019 6,380 6 View
I have purified a monomeric EGFP- His protein with a molecular weight of 30 kDa. But, instead of 30 kDa I am getting a thick band of 60 kDa in SDS-PAGE reducing gel. what is the possible reason...
30 January 2019 5,899 2 View
I have purified a EGFP-His tagged protein which was there in pET15b vector using Ni-NTA column. The concentration obtained was way too high around 5mg/ml. Before the running the SDS-PAGE the...
30 January 2019 7,380 2 View
I am using the Polyomics Integrated Metabolomics Pipeline (PiMP) for metabolite feature analysis. After annotating my data, I have encountered cases where multiple potential metabolites are...
24 July 2024 5,789 0 View
eliminoar articulos que son de tu autoria
05 July 2024 2,107 1 View
Economic factors influence family dynamics.
02 July 2024 6,235 0 View
karma
20 June 2024 6,189 1 View
Hello, From my (naive) understanding, there are several target genes of the canonical Wnt signaling pathway that serve as reliable/validated transcriptional readouts of pathway activity. These...
18 June 2024 4,699 0 View
Es posible observar que mientras que paises como colombia con altos indices de pobreza y desigualdad, abrieron sus fronteras a millones de migrantes venezolanos, por otro lado algunos paises de la...
16 June 2024 236 2 View
I have done caspase-glo 3/7 assay which measures caspase activity after cleavage and generation of luminescent signal produced by luciferase. However,after I got the results, I am not sure how to...
02 June 2024 7,833 2 View
Hello, I duplicated my research account by mistake recently. The recent one shows 2 research publications. I would like to delete it immediately but I don't know how. Can you assist please? I...
28 May 2024 905 0 View
Estoy realizando un curso de Divulgación y Marca Personal del G9 en el que me solicitan hacer una pregunta abierta en ResearchGate.
19 May 2024 4,290 1 View
I am operating a continuous cultivation at fixed carbon and nitrogen concentrations, and under carbon-limitation regime and varying dilution rates from 0.1 to 0.01/hr. Under these conditions all...
13 May 2024 2,510 0 View