18 Questions 18 Answers 0 Followers
Questions related from Puhazh Selvan
I am trying to find the microbial count, Specifically a) total aerobic microbes b) total yeast and mould C) E.Coli and d)Salmonella sp. Could anyone suggest a medium for enumeration of above microbes?
08 August 2018 2,066 12 View
How the flexion point is related to the mechanical strength properties and degradation of a polymer?
02 February 2018 2,626 1 View
The salts should be removed from the solution contains proteins and salts (calcium oxide/ sodium chloride) by diafiltration process. My question is 1) Can we use the ultrafiltration membrane for...
07 July 2017 7,905 9 View
Proteoglycan in liquid samples was estimated by periodic Schiffs assay method (Mantle and Allen, 1978). Whether the presence of free glycosaminoglycan in the sample will interfere the proteoglycan...
07 July 2017 8,280 1 View
The FTIR spectrum of biomass consists protein (60%) and lipids (8%) shows amide I and amide III but does not show the amide II peak at 1510 and 1580 cm-1. What is the possible reason could be?
12 December 2016 6,853 10 View
1) The lipids was extracted and 1H NMR spectra was obtained. In that there is no peak in region 7.0 - 10.0 ppm corresponding for primary and secondary oxidation products . But while doing...
01 January 2016 6,228 2 View
For a chemical reaction, mentioned as conversion is 100% and yield is 66%. what does it mean?
05 May 2015 10,654 9 View
1) I want to concentrate peptides and amino acids present in the liquid. Can i use ammonium sulphate preciptation? Will it works good? 2) Amino acid can be precipitated by changing the pH to...
03 March 2015 3,639 5 View
I want to determine TOC and TKN of solid samples. In our analyzer we can analyze only liquid samples. What is the procedure to hydrolyze/digest the sample? Which acid and its concentration can be...
12 December 2014 4,177 7 View
Usually ammonium sulfate is used to precipitate proteins. Likewise can we precipitate amino acids and peptides using ammonium sulfate or any other means?
12 December 2014 9,989 6 View
Is it possible to extract oil (From seeds) by cloud point extraction method? Can anyone explain the principle behind the cloud point of extraction? In one reference the authors acidified the...
09 September 2014 2,619 1 View
I am going to optimize the medium composition for enzyme production. there are many optimisation method such as RSM, Taguchi Orthogonal array etc... Which method is best for medium optimisation?
05 May 2014 1,598 12 View
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12 December 2013 4,663 3 View
How to screen the antibiofilm activity of a certain chemical which is coated in material used for biomedical applications such as titanium, plastics etc.?
10 October 2013 1,428 4 View
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06 June 2013 938 4 View
05 May 2013 6,964 3 View
Some are used by filter sterilization and some are used by autoclaving. Which is correct?
04 April 2013 5,433 5 View
Is it similar to aerobic bacteria glycerol stock preparation?
04 April 2013 3,593 2 View
