How to screen the antibiofilm activity of a certain chemical which is coated in material used for biomedical applications such as titanium, plastics etc.?
You could simply grow your bacteria in liquid media containing this material. Usually it takes about 3-7 days for a proper biofilm growth depending on the type of bacteria and media. After incubation for suitable number of days you can remove the material from the media, rinse it slowly with sterile deionized water to remove media and debris and scrape off the biofilm. There are various methods to quantify biofilm like the dry weight method, crystal violet staining for visualizing EPS (you can check the absorbence or use microscopy) etc. You can even do this in a microtiter plate to test a range of concentration of your chemical, here is a link which might be useful. http://www.ncbi.nlm.nih.gov/pubmed/17696944
You can compare this with your control to test the anti-biofilm activity.
One thing you need to you need to keep in mind is, chemicals have antibacterial properties and not necessarily antibiofilm activity, it can be tricky to distinguish this. You may see a thinner biofilm but that might be because it is lethal to the bacteria, so it is important to check the inhibitory effect first before doing the biofilm assay. At a particular concentration if the chemical gives same turbidity (growth) as the control but less biofilm then the experiment might be valid.
hi! I would suggest to use those surfaces with the chemical coatings as subtracts for biofilm formation, using as control the same materials without the chemicals. Use it in simple small coupons it would be easy to grow the biofilms in microtiter plates (48 or 24 wells). After the biofilm grow (24h is enough), ressuspend the coupon in a physiological solution (saline or phosphate buffer) and then take samples for that for serial dilution and plate couting. You can then compare the number of CFU in each material+chemical.
You can see more information about a similar assay on my paper: http://www.sciencedirect.com/science/article/pii/S0964830513002242
hi
In most of the papers the antibiofilm activity is measured using microtitre plate. but i cant incubate my material in 96 well microtitre plate. could you suggest some reference for the experiment that carried out in material incubate in the petri dish to measure antibiofilm activity?
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