It depends on what type of waste water you are using? What is your aim on treatment, are you interested in COD removal, or nutrient removal? are you producing any valuable end product? The over all wok depends on these factors.
you have to control the parameters that constrain the growth of bacteria. It is important to know the characteristics of the waste you have to try and what features you expect to have in the treated effluent.
For example, work at the plant where the waste is industrial, large quantities of ammonia. It is aspirated to remove nitrates being transformed by nitrifying bacteria through an aerobic system and then pass it to nitrogen gas through anaerobic system acting denitrifying bacteria.
For waste water treatment using bacteria you should first find out that at what organic loading your bacteria is performing well. pH, temperature and aeration also plays important role so you have to consider all these factors also.
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