Is there any standard protocol for tris buffered phenol preparation?

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What is the relationship between the threshold voltage and the device's noise performance, particularly in terms of flicker noise and thermal noise?

The threshold voltage (Vth) of a MOS device plays a crucial role for its operation. At the same time, noise is an intrinsic factor. So how noise (flicker or thermal) change with the change in...

29 July 2024 3,246 0 View

Is it possible to dock more than one drug in the same active site or pocket of a protein to study synergistic/antagonistic/additive properties?

I wanted to know whether we can observe the synergistic/antagonistic/additive properties of combinations or mixtures of compounds through docking analysis. But during docking preparation any...

28 July 2024 7,413 6 View

What is the solvent system required to run tlc for calixarenes?

If you don't understand my question let me know

21 July 2024 563 1 View

Sample size for qualitative study?

I am working on scale development in behavioral finance by undertaking a mixed-method approach using the exploratory sequential design. The phenomenon has diverse meanings in existing literature...

20 July 2024 9,153 11 View

How to convert available phosphorus to total phosphorus?

I need to convert Available phosphorus into total phosphorus

18 July 2024 6,799 3 View

Can it is possible to find the cleaved sequence when a protein cleaved by a heamaglutanin protease (HA/P) by any bioinformatics tools?

Bioinformatics tools like peptide cutter

15 July 2024 6,453 1 View

Can we progress towards defining obesity based on body fat content?

The burden of obesity is enormous. The greater challenge lies in accurately diagnosing obesity at the right time, reflecting the true increase in visceral fat. BMI may not accurately represent...

14 July 2024 8,771 4 View

How does high humidity affect the growth and development of crops?

10 July 2024 7,258 20 View

In what ways does high humidity impact the pollination process in agricultural plants?

10 July 2024 7,513 13 View

Do the parts of the body that are situated at a longer distance from the central nervous system have the least control & coordination comparatively?

What would be the effect of distance from CNC cum Heart on the structure and function of a particular part of the human body? Do short people have evolutionary advantages to control body parts in...

09 July 2024 2,052 3 View

Help me to identify these fungal slides?

These fungal slides are isolated from cultured Bread mold on PDA media and smear is treated with Lacto phenol Blue dye ..

13 July 2024 9,701 1 View

What is the exact procedure and materials for converting from ester to carbolic acid by base hydrolysis??

11 July 2024 4,645 3 View

Lipopolysaccharide (LPS) extraction problem?

Hello, all I am currently extracting LPS from E. coli MG1655 and its mutant strains. I followed several different protocols (phenol methods or phenol+diethy ether methods), but I couldn't see...

25 June 2024 7,568 1 View

Kindly help me to identify this fungal slide ?

fungal inoculum treated with lacto phenol blue and observed under microscope anyone help me to identify and share some information regarding this fungal slide

07 June 2024 9,647 3 View

Extraction RNA with miRVANA kit?

Hello everyone, I would like to know if any of you ever tried to assess the yeld of RNA extraction with the mirVana™ miRNA Isolation Kit, without phenol from ThermoFisher. I am experincing some...

06 June 2024 5,715 4 View

Why did my hydrogel suddenly become acidic without changing the conditions in which it was made?

I have been making hydrogels regularly using 10X DMEM (phenol red indicator), Ultrapure water, 1N NaOH, and 3.41 mg/ml Collagen (suspended with acetic acid). We have used the same calculations for...

29 May 2024 4,655 0 View

Total phenol content estimation?

The addition of Na2CO3 in TPC estimation creates an alkaline medium for phenols to get unstable, however whenever I add 2 ml of 20% of Na2CO3 to 1 ml of gallic acid extract and 0.5 ml of FC...

14 May 2024 5,478 2 View

How do I isolate Lipase producing Fungi from spoilt Sheabutter and Ghee?

I prepared an enrichment media making use of the two samples spoilt Sheabutter and Ghee (differently) and it was cultured on Olive oil agar using pour plate method. The organisms were subcultured...

05 May 2024 5,795 1 View

What can I do for DNA extraction of bacteria?

It is well known that SDS and CTAB is a good method for removing protein in DNA extraction, but i am doing extraction of dna of pseudomonas bacteria and i didn't see any good results, although i...

09 April 2024 1,908 2 View

Why generally we prefer phenol chloroform extraction method for DNA purification as i always face difficulty in getting good concentration why?

phenol chloroform extraction method is used for purification of nucleic acids DNA/RNA

03 April 2024 8,268 0 View