Hi everyone,
I want to do estimate antioxidant enzymes activity of crude protein extract obtained from salt stress induced cotton seedlings. Can anyone give me standard protocols for SOD, ascorbate peroxidase, catalase and other antioxidant chemicals? Thanks in advance.
There are numerous enzymatic and non-enzymatic antioxidants to study in plants. These papers have helped me.
http://www.ias.csic.es/janc/sci_papers/physiolmolecplantpathol_61_325_02.pdf
http://www.scielo.br/pdf/bjpp/v22n3/v22n3a01.pdf
http://www.sciencedirect.com/science/article/pii/S0098847204000292
Dear Prem,
As your friend wrote you there are several methods for them, but i want to tell you about new method for testing of Total antioxidant capacity of your samples with "FRAP test".
The ferric reducing antioxidant power (FRAP) of your samples will assessed in each experimental group. it is a simple test. you can find this methods from my recent articles specially in this:
The Hepatoprotection Provided by Taurine and Glycine against Antineoplastic Drugs Induced Liver Injury in an Ex Vivo Model of Normothermic Recirculating Isolated Perfused Rat Liver.
You can test it in plant specimens, indeed.
Regards,
Mehdi
Dear Prem,
The above method given by Mehdi Sir is for invivo antioxidant activity in animals but i suggest you the below references for your plant antioxidant estimations...go through them and i hope you find your answer!!!
Determination of SOD, POD, PPO and CAT Enzyme Activities in Rumex obtusifolius L. Annual Research & Review in Biology 11(3): 1-7, 2016; Article no.ARRB.29809
www.sciencedomain.org
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