I have a peptide I would like to quantify with 214 nm abrobance. Is there an algorithm (or a rule of thumb,) to predict what the absorbance should be like there for 280nm?
Dear Pavel V Shliaha,
I suggest you to see links and attached files on topic.
In Vitro and In Silico Approaches to Generating and Identifying ... - MDPI
https://www.mdpi.com/1660-3397/17/4/204/pdf
Extinction Coefficient Determination of Proteins - Bio-Synthesis
https://www.biosyn.com/.../How-is-Extinction-Coefficient-Determi...
Best regards
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