I developed a suitable plasmid for tomato, and I am trying to understand mathematics behind the technique. I need an example file to proceed.
You should construct a standard curve with a known copy nr, and then interpolate your sample Cp.
Normally the people use a UV determined concentration of plasmid containing the gene of interest, and serial dillutions for the standard curve.
http://www.ncbi.nlm.nih.gov/pubmed/23308234
I hope it will be helpful.
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