The native protein Aldolase A (mol. Wt. 39kDa) was showing the broadening of a peak in MALDI. Sometimes the spectra were too poor to analyze.
Hi,
It would be helpful to include an example of a MALDI spectrum you're referring to, but first thing that comes to mind is: desalting (via diafiltration) of the protein sample?
And is the aldolase A preparation you're looking at pure (>90%), or contaminated with other proteins/isoforms?
Hope this helps!
Hi Neelesh,
Since the performance of a protein spectra signal by MALDI-TOF analysis could be affected by different parameters, It would help you provide more information: Sample preparation used, matrix etc.
Nevertheless I send you our receipt in case it can be of help: desalt proteins with C4 Reverse Pahse ZipTips, and elute proteins from the resine usin Synapinic acid (10 mg/ml) in 70 ACN:30 0.1% TFA.
Good luck!
Hello,
For MALDI it is very important crystallization, so be careful with salts, also you have to consider the sample concentration.
Sometimes it is helpful to control manually the laser power/position, increasing the quantity of spectra acquired, modifying the sets in the tof instrument method due they are several settings for large proteins instead peptides.
- Badges
- Science topic
- Similar topics
- Solid State Physics
- Devices