Dear Mohit,

The attached recent publication might be helpful and fulfill your requirements without the need to purchase expensive kits:

Dissecting the cell to nucleus, perinucleus and cytosol

Tattym E. Shaiken1 & Antone R. Opekun2

1

Department of Molecular and Cellular Oncology, University of Texas M. D. Anderson Cancer Center, Houston, TX 77030, USA,

2

Departments of Medicine & Pediatrics G.I. & S.A.H.S. Baylor College of Medicine-McNair Faculty Center A10.019 One Baylor

Plaza (GI Medicine MS901), Houston, Texas 77030, USA.

Cells have been described under the microscope as organelles containing cytoplasm and the nucleus. However, an unnoted structure exists between the cytoplasm and the nucleoplasm of eukaryotic cells. In addition to the nuclear envelope, there exists a perinuclear region (PNR or perinucleus) with unknown

composition and function. Until now, an investigation of the role of the perinucleus has been restricted by the absence of a PNR isolation method. This manuscript describes a perinucleus isolation technique on the basis of its unique compact organization. The perinucleus was found to contain approximately 15 to 18% of

the total proteins of the mammalian cell, almost half of the proteins of nuclei. Using four different normal and cancer cell lines, it was shown that the composition of PNR is highly dynamic. Application of the method showed that translocation of the p53 tumor-suppressor protein to the perinucleus in immortalized MEF cells is correlated with the translocation of p53-stabilizing protein, nucleophosmin (B23), to the PNR. Herein, the concept of the perinuclear region is advanced as a formal, identifiable structure. The roles of the perinucleus in maintaining genome integrity, regulation of gene expression and understanding of malignant transformation are discussed.

Hoping this will be helpful,

Rafik

Thanks Rafik for the advice