How to recrystallization amine compound and it is not soluble in common organic solvents ?

How to recrystallization amine compound and it is not soluble in common organic solvents ?

19 January 2021 671 3 View

What is the ACTUAL COST OF A PUBLICATION??

Hi. It's 2021 January. Recently, I got to know that Elsevier has converted around 160 journals into some "Transformative Journals" and follow the recommendations under "Plan S"...

19 January 2021 9,985 2 View

What if an author has concluded wrong facts in his article and published in Elsevier ?

The question is that in what form of publication we can show true facts and prove the wrong facts invalid ?

29 December 2020 9,727 7 View

What is the difference between filler and fiber?

Explain in correct manner

28 December 2020 5,199 2 View

What should be done if someone 100% copied our article and published in a predatory journal ?

28 December 2020 8,982 10 View

Advantages and Disadvantages of Submitting Article in Special Issues of a Journal ?

What are those ?

21 December 2020 7,757 4 View

What is U matrix for Hosking & Wallis discordancy test based on L-comoments?

Hello everyone I would compute Hosking & Wallis discordancy test based on L-comoments for multivariate Regional Frequency Analysis. Please help me by answer to these questions. 1- Is that...

28 November 2020 8,152 2 View

In microscopy why "smaller the aperture, greater the depth of field"?

12 October 2020 3,636 2 View

How is the resolution of an image affected by the NA of the lens in microscopy?

NA= Numerical Aperture

12 October 2020 2,514 2 View

Which will have a larger Depth of Field: A Light Microscope or an Electron Microscope? Why/How?

The depth of field is the range of distance along the optical axis in which the specimen can move without detectable loss of sharpness.

11 October 2020 4,623 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

What is the proper in vitro culturing method to get information about the activation of innate immune cells by VLPs?

Dear All, I am interested in studying the response of immune cells against virus-like particles (VLPs). I used the PBMC in vitro assay and treat it with the VLP using PHA as a positive ctrl while...

16 February 2021 1,337 2 View

Why does flow deformation occur at the test line and control line on nitrocellulose membranes (LFIA)?

I use 1% BSA in 10 mM phosphate buffer (pH 7.4) solution when striping my nitrocellulose membranes with antigens and antibodies. However, I always observe deformed flow when my sample or running...

16 February 2021 4,224 3 View

Can the viability assays (ex: MTT/CCK-8) measure cell proliferation? or are they solely viability assays?

I want to conduct a proliferation assay to test a chemotherapy combination on a cancer cell line. At first, I thought about the MTT assay. But now, all sources are saying that these do not measure...

14 February 2021 3,142 7 View

GST assay in microplate?

I'd like to know if anyone can provide me with a protocol for traditional GST enzymatic assay (with GSH and CDNB as substrates) in a 96 well microplate, for cell line and/or C.elegans...

11 February 2021 1,328 3 View

What lysis buffer is compatible with Bradford assay?

Hello! I am planning to use Bradford assay to measure protein content in PBMCs lysates. The problem is that Bradford assay doesn't seem to be compatible with Tris-based buffers or SDS. Are there...

10 February 2021 1,797 5 View

For PC-3 cell line. How long before irradiation do I have to synchronize my cells to G0/G1?

I am trying to do a clonogenic assay for PC-3 after irradiation. I have to synchronize my cells by serum starvation but there's a optimal time I have to do that before irradiation. been looking in...

09 February 2021 9,480 1 View

What is best technique to quantify necrotic cell death of specific cell populations?

I currently using FACS (Aurora Cytek) to quantify necrotic Gr1+ cell death from the spleens of immune competent mice. I target Gr1+ cells in the spleens of tumor-bearing mice using a photodynamic...

08 February 2021 733 6 View